Heterotypic tumor models through freeform printing into photostabilized granular microgels.
Heterotypic tumor models through freeform printing into photostabilized granular microgels.
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DOI：
10.1039/d1bm00574j
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发表时间：
2021-06-15
影响因子：
6.6
通讯作者：
Kilian KA
中科院分区：
文献类型：
--
作者：
Molley TG;Jalandhra GK;Nemec SR;Tiffany AS;Patkunarajah A;Poole K;Harley BAC;Hung TT;Kilian KA
组织微环境包含多种细胞类型、基质和血管结构的复杂分类，其难以在体外重建。在这里，我们展示了通过在载有细胞的微凝胶基质内直接写入血管通道和肿瘤细胞聚集体而形成的模型肿瘤微环境。光交联微凝胶提供对局部和全局力学的控制，同时使几乎任何细胞类型的整合成为可能。将Pluronic牺牲油墨直接写入基质细胞-微凝胶悬浮液中用于形成用于内皮化的血管结构，然后打印黑色素瘤聚集体。肿瘤细胞作为空间位置的函数迁移到原型血管中，从而提供了侵入潜力的测量。灌注通道与多种空间定义的细胞类型的整合为建模发展和疾病提供了新的途径，为基础研究和药物开发工作提供了空间。
The tissue microenvironment contains a complex assortment of multiple cell types, matrices, and vessel structures, which is difficult to reconstruct in vitro. Here, we demonstrate model tumor microenvironments formed through direct writing of vasculature channels and tumor cell aggregates, within a cell-laden microgel matrix. Photocrosslinkable microgels provide control over local and global mechanics, while enabling the integration of virtually any cell type. Direct writing of a Pluronic sacrificial ink into a stromal cell-microgel suspension is used to form vessel structures for endothelialization, followed by printing of melanoma aggregates. Tumor cells migrate into the prototype vessels as a function of spatial location, thereby providing a measure of invasive potential. The integration of perfusable channels with multiple spatially defined cell types provides new avenues for modelling development and disease, with scope for both fundamental research and drug development efforts.
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