Insights into the skin microbiome of sickle cell disease leg ulcers.
Insights into the skin microbiome of sickle cell disease leg ulcers.
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DOI：
10.1111/wrr.12924
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Grice EA
中科院分区：
文献类型：
--
作者：
Byeon J;Blizinsky KD;Persaud A;Findley K;Lee JJ;Buscetta AJ;You S;Bittinger K;Minniti CP;Bonham VL;Grice EA
据估计，在北美镰状细胞病(SCD)患者中，腿部溃疡的发生率为1%-10%。它们的病理生理学仍不明确，但与其他慢性伤口一样，人们假设微生物环境或微生物群对它们的愈合和临床结果有贡献。本研究利用16S核糖体RNA(RRNA)基因测序首次描述了SCD小腿溃疡的微生物群及其与临床因素的关系。在对42个溃疡的横断面分析中，我们恢复了与其他慢性创面类似的微生物图谱，其中厌氧菌和机会致病菌占主导地位，包括葡萄球菌、棒状杆菌和芬格氏菌。溃疡分为两类：一类以葡萄球菌为主，表面积较小；另一类表现出较大的类群多样性和较大的表面积。我们还发现，卟啉单胞菌的相对丰度与血红蛋白水平负相关，血红蛋白水平是SCD的关键临床严重性指标，Finegoldia的相对丰度与CD19+B细胞计数负相关。最后，在有SCD腿部溃疡病史的人的完整皮肤中，棒状杆菌与乳杆菌和葡萄球菌与乳杆菌的比例升高，而在无溃疡病史的人中，乳杆菌与芽孢杆菌的比例升高。对皮肤微生物群与SCD溃疡病理生理学的关系的研究可以为这一鲜为人知的慢性创面的临床指南提供参考，并增进对皮肤微生物群在延迟伤口愈合中所起作用的更广泛的理解。
Leg ulcers are estimated to occur in 1%–10% of North American patients with sickle cell disease (SCD). Their pathophysiology remains poorly defined, but as with other chronic wounds, it is hypothesised that the microbial milieu, or microbiome, contributes to their healing and clinical outcomes. This study utilises 16S ribosomal RNA (rRNA) gene sequencing to describe, for the first time, the microbiome of the SCD leg ulcer and its association with clinical factors. In a cross‐sectional analysis of 42 ulcers, we recovered microbial profiles similar to other chronic wounds in the predominance of anaerobic bacteria and opportunistic pathogens including Staphylococcus, Corynebacterium, and Finegoldia. Ulcers separated into two clusters: one defined by predominance of Staphylococcus and smaller surface area, and the other displaying a greater diversity of taxa and larger surface area. We also find that the relative abundance of Porphyromonas is negatively associated with haemoglobin levels, a key clinical severity indicator for SCD, and that Finegoldia relative abundance is negatively associated with CD19+ B cell count. Finally, ratios of Corynebacterium:Lactobacillus and Staphylococcus:Lactobacillus are elevated in the intact skin of individuals with a history of SCD leg ulcers, while the ratio of Lactobacillus:Bacillus is elevated in that of individuals without a history of ulcers. Investigations of the skin microbiome in relation to SCD ulcer pathophysiology can inform clinical guidelines for this poorly understood chronic wound, as well as enhance broader understanding about the role of the skin microbiome in delayed wound healing.
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影响因子：
2.7
作者：
Gardiner M;Vicaretti M;Sparks J;Bansal S;Bush S;Liu M;Darling A;Harry E;Burke CM
通讯作者：
Burke CM
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1016/j.biocel.2006.01.010
发表时间：
2006-01-01
影响因子：
4
作者：
Mack, A. Kyle;Kato, Gregory J.
通讯作者：
Kato, Gregory J.
影响因子：
6.5
作者：
Clare, A;FitzHenley, M;Serjeant, GR
通讯作者：
Serjeant, GR
影响因子：
2.9
作者：
Kirsner, Robert S.
通讯作者：
Kirsner, Robert S.