Molecular genetic evidence for polyandry in Ascaris suum

Molecular genetic evidence for polyandry in Ascaris suum
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猪蛔虫一妻多夫的分子遗传学证据

DOI:
10.1007/s00436-010-2116-3
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发表时间:
2011-03
影响因子:
2
通讯作者:
Peng, Weidong
Peng, Weidong
中科院分区:
医学3区
文献类型:
--
作者:
Zhou, Chunhua;Yuan, Keng;Tang, Xiaoli;Hu, Ningyan;Peng, Weidong

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本研究的目的是确定单个雌性猪蛔虫是否可以与多个雄性交配。采用七个性别连锁微卫星标记并进行父系遗传分析。总共对三名单身女性的 62 名后代进行了筛查,并使用等位基因计数方法和 GERUD 2.0 版程序确定了每个家庭中父亲的数量。七个与性别相关的微卫星位点表现出高度多态性,并显示抽样家庭中三分之一的家庭(等位基因计数)和三分之二的家庭(GERUD)至少有两个父亲(2-6),表明猪猪的雌性可以与多个雄性交配。这些发现为雌性A. suum一妻多夫制提供了首个分子遗传学证据,并为进一步研究一妻多夫对群体遗传参数、寄生虫群体遗传多样性、不同宿主物种感染潜力以及耐药性传播速度的影响奠定了基础。
The aim of this study was to determine whether single Ascaris suum female could mate with multiple males. Seven sex-linked microsatellite markers were employed and paternal genetic analyses were conducted. Totally, 62 offspring individuals from three single females were screened, and the numbers of fathers in each family were determined using allele counting methods and the program GERUD, version 2.0. The seven sex-linked microsatellite loci showed high polymorphism and revealed that one out of three families (allele counts) and two out of three families (GERUD) of the sampled families had at least two sires (2–6), indicating that females of A. suum can mate with multiple males. These findings provide the first molecular genetic evidence for polyandry of female A. suum and lay a foundation for further studies on the impacts of polyandry on population genetic parameters, the parasite population’s genetic diversity, the potential for infection of different host species, and for the rate of spread of drug resistance.
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