Microbiome composition within a sympatric species complex of intertidal isopods (Jaera albifrons).
Microbiome composition within a sympatric species complex of intertidal isopods (Jaera albifrons).
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DOI：
10.1371/journal.pone.0202212
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Piertney SB
中科院分区：
文献类型：
--
作者：
Wenzel MA;Douglas A;Piertney SB
微生物组对宿主生态进化动态的影响日益被认识，这促进了“全基因组”作为一个完整的宿主-共生进化实体的观点。例如，性别比例扭曲的生殖寄生虫，如沃尔巴克氏体，是无脊椎动物生殖过程的关键驱动因素，最近的工作强调了微生物组组合对宿主交配行为和发育不相容的新影响，这些影响支持或加强了生殖隔离过程。然而，在自然种群中检测全基因组及其生态进化效应是具有挑战性的，因为微生物组的组成受到环境因素的很大影响。在这里，我们说明了这些挑战在潮间带等足类的同域物种复杂（Jaera albirons spp.）普遍存在性别比例扭曲和生态和行为生殖隔离机制。我们对苏格兰东北部两个海岸春夏采集的雄性和雌性细菌的16S rRNA基因进行了深度测序，并研究了微生物组的组成，特别关注生殖寄生虫。所有物种的微生物组具有多样性（380万reads中共有3317个独特序列），主要由海洋潮间带典型的变形菌门和拟杆菌门类群组成，特别是弧菌，但我们发现很少有生殖寄生虫沃尔巴克氏体，立克次体，螺旋体和基数虫的证据，这表明性别比例扭曲的其他原因。尽管如此，样本间微生物组组成差异的很大一部分可以用性别（14.1%）、地理（26.9%）和季节（39.6%）方差成分来解释。由于缺乏生殖寄生虫，物种组合的短暂性和巨大的环境可变性，这种性信号的功能相关性很难确定。这些结果确立了白斑Jaera物种复合体是一个有趣的系统，用于研究微生物组对生殖过程和物种形成的影响，并强调了在动态和复杂环境中对微生物组组成进行快照分析的困难。
The increasingly recognised effects of microbiomes on the eco-evolutionary dynamics of their hosts are promoting a view of the “hologenome” as an integral host-symbiont evolutionary entity. For example, sex-ratio distorting reproductive parasites such as Wolbachia are well-studied pivotal drivers of invertebrate reproductive processes, and more recent work is highlighting novel effects of microbiome assemblages on host mating behaviour and developmental incompatibilities that underpin or reinforce reproductive isolation processes. However, examining the hologenome and its eco-evolutionary effects in natural populations is challenging because microbiome composition is considerably influenced by environmental factors. Here we illustrate these challenges in a sympatric species complex of intertidal isopods (Jaera albifrons spp.) with pervasive sex-ratio distortion and ecological and behavioural reproductive isolation mechanisms. We deep-sequence the bacterial 16S rRNA gene among males and females collected in spring and summer from two coasts in north-east Scotland, and examine microbiome composition with a particular focus on reproductive parasites. Microbiomes of all species were diverse (overall 3,317 unique sequences among 3.8 million reads) and comprised mainly Proteobacteria and Bacteroidetes taxa typical of the marine intertidal zone, in particular Vibrio spp. However, we found little evidence of the reproductive parasites Wolbachia, Rickettsia, Spiroplasma and Cardinium, suggesting alternative causes of sex-ratio distortion. Notwithstanding, a significant proportion of the variance in microbiome composition among samples was explained by sex (14.1 %), nested within geographic (26.9 %) and seasonal (39.6 %) variance components. The functional relevance of this sex signal was difficult to ascertain given the absence of reproductive parasites, the ephemeral nature of the species assemblages and substantial environmental variability. These results establish the Jaera albifrons species complex as an intriguing system for examining the effects of microbiomes on reproductive processes and speciation, and highlight the difficulties associated with snapshot assays of microbiome composition in dynamic and complex environments.
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影响因子：
3.7
作者：
Degli Esposti M;Martinez Romero E
通讯作者：
Martinez Romero E
影响因子：
9.8
作者：
Brooks AW;Kohl KD;Brucker RM;van Opstal EJ;Bordenstein SR
通讯作者：
Bordenstein SR
影响因子：
9.8
作者：
Bordenstein SR;Theis KR
通讯作者：
Theis KR
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1098/rspb.1998.0402
发表时间：
1998-06-22
影响因子：
4.7
作者：
Bouchon, D;Rigaud, T;Juchault, P
通讯作者：
Juchault, P