A mechanical signal transmitted by the flagellum controls signalling in Bacillus subtilis.
A mechanical signal transmitted by the flagellum controls signalling in Bacillus subtilis.
复制标题
鞭毛传递的机械信号控制枯草芽孢杆菌的信号传导。
DOI：
10.1111/mmi.12342
复制
发表时间：
2013-10
影响因子：
3.6
通讯作者：
Stanley-Wall NR
中科院分区：
文献类型：
--
作者：
Cairns LS;Marlow VL;Bissett E;Ostrowski A;Stanley-Wall NR
在自然环境中，细菌主要作为生物膜的一部分粘附在表面上。虽然生物膜组装所需的许多组分是已知的，但微生物感知和响应与表面接触的机制知之甚少。枯草芽孢杆菌是生物膜形成的革兰氏阳性模型。DegS-DegU双组分系统控制B. subtilis中的几种多细胞行为，包括生物膜形成。在这里，我们确定了B. subtilis鞭毛作为激活DegS-DegU调节途径的机械传感器。通过鞭毛定子基因motB的缺失或突变来抑制鞭毛旋转，导致degU转录和DegU β P驱动的过程（即外切蛋白酶产生和聚-γ-dl-谷氨酸生物合成）增加。类似地，通过接合鞭毛离合器或通过用抗体拴系鞭毛来抑制鞭毛旋转也促进了degU转录的增加，这反映了细胞中DegU β P水平的增加。总的来说，这些研究结果强烈表明，抑制鞭毛旋转作为一个机械触发激活DegS-DegU双组分信号转导系统。我们假设，鞭毛旋转的抑制可以作为一个机械触发器，激活细菌信号转导级联在许多能动细菌接触表面。
In the natural environment bacteria predominantly live adhered to a surface as part of a biofilm. While many of the components needed for biofilm assembly are known, the mechanism by which microbes sense and respond to contact with a surface is poorly understood. Bacillus subtilis is a Gram-positive model for biofilm formation. The DegS–DegU two-component system controls several multicellular behaviours in B. subtilis, including biofilm formation. Here we identify the B. subtilis flagellum as a mechanosensor that activates the DegS–DegU regulatory pathway. Inhibition of flagellar rotation by deletion or mutation of the flagellar stator gene, motB, results in an increase in both degU transcription and DegU∼P driven processes, namely exoprotease production and poly-γ-dl-glutamic acid biosynthesis. Similarly, inhibition of flagellar rotation by engaging the flagellar clutch or by tethering the flagella with antibodies also promotes an increase in degU transcription that is reflective of increased DegU∼P levels in the cell. Collectively, these findings strongly indicate that inhibition of flagellar rotation acts as a mechanical trigger to activate the DegS–DegU two-component signal transduction system. We postulate that inhibition of flagellar rotation could function as a mechanical trigger to activate bacterial signal transduction cascades in many motile bacteria upon contact with a surface.
登录
查看更多内容
影响因子：
3.6
作者：
Gode-Potratz CJ;Kustusch RJ;Breheny PJ;Weiss DS;McCarter LL
通讯作者：
McCarter LL
影响因子：
64.5
作者：
BURBULYS, D;TRACH, KA;HOCH, JA
通讯作者：
HOCH, JA
影响因子：
3.2
作者：
Diethmaier, Christine;Pietack, Nico;Stuelke, Joerg
通讯作者：
Stuelke, Joerg
影响因子：
11.4
作者：
Chai, Yunrong;Norman, Thomas;Losick, Richard
通讯作者：
Losick, Richard
影响因子：
3.2
作者：
Calvio, Cinzia;Osera, Cecilia;Galizzi, Alessandro
通讯作者：
Galizzi, Alessandro