Digital barcodes of suspension array using laser induced breakdown spectroscopy.

Digital barcodes of suspension array using laser induced breakdown spectroscopy.
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使用激光诱导击穿光谱的悬浮阵列数字条形码

DOI:
10.1038/srep36511
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发表时间:
2016-11-03
期刊:
影响因子:
4.6
通讯作者:
Shen Z
Shen Z
中科院分区:
综合性期刊3区
文献类型:
--
作者:
He Q;Liu Y;He Y;Zhu L;Zhang Y;Shen Z

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我们提出了一种基于激光诱导击穿光谱(LIBS)的悬浮阵列编码方法,使条形码从模拟到数字化。作为数字光学条码的基础,采用自组装封装法制备了纳米晶编码微球。我们确认基于LIBS的编码方法的数字复用是可行的,因为微球可以用波长的直接读出数据编码,并且该方法可以避免条形码和分析物标签之间的荧光信号串扰,这导致当前荧光标记物编码方法在准确性和稳定性方面的整体优势。该演示提高了多重检测和精确筛选的能力,拓展了悬浮阵列在生命科学中更广泛的应用。
We show a coding method of suspension array based on the laser induced breakdown spectroscopy (LIBS), which promotes the barcodes from analog to digital. As the foundation of digital optical barcodes, nanocrystals encoded microspheres are prepared with self-assembly encapsulation method. We confirm that digital multiplexing of LIBS-based coding method becomes feasible since the microsphere can be coded with direct read-out data of wavelengths, and the method can avoid fluorescence signal crosstalk between barcodes and analyte tags, which lead to overall advantages in accuracy and stability to current fluorescent multicolor coding method. This demonstration increases the capability of multiplexed detection and accurate filtrating, expanding more extensive applications of suspension array in life science.
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