Gut bacterial and fungal communities of the domesticated silkworm (Bombyx mori) and wild mulberry-feeding relatives.
Gut bacterial and fungal communities of the domesticated silkworm (Bombyx mori) and wild mulberry-feeding relatives.
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家蚕（Bombyx mori）和野生桑蚕近缘种的肠道细菌和真菌群落
DOI：
10.1038/s41396-018-0174-1
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Shao Y
中科院分区：
文献类型：
--
作者：
Chen B;Du K;Sun C;Vimalanathan A;Liang X;Li Y;Wang B;Lu X;Li L;Shao Y
家蚕是一种重要的蚕丝生产者，也是基础研究和应用研究的有力实验模型。与其他动物类似，家蚕肠道内生活着丰富的微生物;然而，令人惊讶的是，迄今为止，这种模式昆虫的微生物群尚未得到很好的表征。在这里，我们全面描述了家蚕及其野生亲属的肠道微生物群。与桑树饲养蛾Acronicta major和Diaphania pyloalis的比较分析显示，尽管经过数千年的驯化，但家蚕肠道微生物群高度多样但独特，并且在总（基于DNA）和活性（基于RNA）细菌种群中具有阶段特异性特征，主要由变形菌门，厚壁菌门，放线菌门和拟杆菌门。大多数真菌序列被分配到子囊菌门和担子菌门。环境因素，包括饮食和人为操作（产卵），可能会影响家蚕肠道组成。尽管缺乏空间变化沿着肠道，微生物群落的变化是明显的，在音乐会与主机的发展变化之间的早期和晚期龄。我们的研究结果表明，家蚕的肠道微生物群在整个发育过程中组装成越来越相同的群落，这与来自相同生态位的其他食桑鳞翅目昆虫的群落有很大不同，突出了宿主特异性对微生物协会的影响以及这些群落在宿主生物学中发挥的潜在作用。
Bombyx mori, the domesticated silkworm, is of great importance as a silk producer and as a powerful experimental model for the basic and applied research. Similar to other animals, abundant microorganisms live inside the silkworm gut; however, surprisingly, the microbiota of this model insect has not been well characterized to date. Here, we comprehensively characterized the gut microbiota of the domesticated silkworm and its wild relatives. Comparative analyses with the mulberry-feeding mothsAcronicta majorandDiaphania pyloalisrevealed a highly diverse but distinctive silkworm gut microbiota despite thousands of years of domestication, and stage-specific signatures in both total (DNA-based) and active (RNA-based) bacterial populations, dominated by the phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Most fungal sequences were assigned to the phyla Ascomycota and Basidiomycota. Environmental factors, including diet and human manipulation (egg production), likely influence the silkworm gut composition. Despite a lack of spatial variation along the gut, microbial community shifts were apparent between early instars and late instars, in concert with host developmental changes. Our results demonstrate that the gut microbiota of silkworms assembles into increasingly identical community throughout development, which differs greatly from those of other mulberry-feeding lepidopterans from the same niche, highlighting host-specific effects on microbial associations and the potential roles these communities play in host biology.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
DOI：
10.1099/ijsem.0.001200
发表时间：
2016-09-01
影响因子：
2.8
作者：
Aydogan, Ebru L.;Busse, Hans-Juergen;Glaeser, Stefanie P.
通讯作者：
Glaeser, Stefanie P.
影响因子：
11
作者：
Brettar, Ingrid;Christen, Richard;Hoefle, Manfred G.
通讯作者：
Hoefle, Manfred G.
影响因子：
11
作者：
Adams, Aaron S.;Jordan, Michelle S.;Raffa, Kenneth F.
通讯作者：
Raffa, Kenneth F.
DOI：
10.1673/031.010.10701
发表时间：
2010
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Anand AA;Vennison SJ;Sankar SG;Prabhu DI;Vasan PT;Raghuraman T;Geoffrey CJ;Vendan SE
通讯作者：
Vendan SE