Alcohol abrogates human norovirus infectivity in a pH-dependent manner.
Alcohol abrogates human norovirus infectivity in a pH-dependent manner.
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DOI：
10.1038/s41598-020-72609-z
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发表时间：
2020-09-28
影响因子：
4.6
通讯作者：
Uematsu S
中科院分区：
文献类型：
--
作者：
Sato S;Matsumoto N;Hisaie K;Uematsu S
酒精类消毒剂广泛用于微生物的消毒，特别是那些引起包括病毒在内的传染病的微生物。然而，由于酒精的杀菌机制是分解脂肪，基于酒精的消毒剂似乎对非包膜病毒（如诺如病毒）的作用最小。由于目前尚无人诺如病毒（HuNoV）的体外培养方法，本文以鼠诺如病毒和猫杯状病毒作为HuNoV的替代物，分析了消毒液的效果。因此，这些消毒剂及其使用条件是否对人体免疫缺陷病毒有效仍不得而知。在这项研究中，我们报告了乙醇或异丙醇单独可以充分抑制人类ipsc来源的肠上皮细胞中GII.4基因型HuNoV的复制。此外，pH调节和盐析可能有助于酒精对其他HuNoV基因型的杀病毒作用，并分别抵消有机物污染的障碍。因此，与次氯酸钠类似，含有电解质的酒精类消毒剂可用于灭活HuNoV。
Alcohol-based disinfectants are widely used for the sanitization of microorganisms, especially those that cause infectious diseases, including viruses. However, since the germicidal mechanism of alcohol is lipolysis, alcohol-based disinfectants appear to have a minimal effect on non-enveloped viruses, such as noroviruses. Because there is no cultivation method for human norovirus (HuNoV) in vitro, murine norovirus and feline calicivirus have been used as surrogates for HuNoV to analyze the efficacy of disinfectant regents. Therefore, whether these disinfectants and their conditions are effective against HuNoVs remain unknown. In this study, we report that ethanol or isopropanol alone can sufficiently suppress GII.4 genotype HuNoV replication in human iPSC-derived intestinal epithelial cells. Additionally, pH adjustments and salting-out may contribute toward the virucidal effect of alcohol against other HuNoV genotypes and cancel the impediment of organic substance contamination, respectively. Therefore, similar to sodium hypochlorite, alcohol-based disinfectants containing electrolytes can be used for HuNoV inactivation.
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影响因子：
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