Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation.
Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation.
复制标题
DOI：
10.3201/eid2408.180126
复制
发表时间：
2018-08
影响因子：
11.8
通讯作者：
Vinjé J
中科院分区：
文献类型：
--
作者：
Costantini V;Morantz EK;Browne H;Ettayebi K;Zeng XL;Atmar RL;Estes MK;Vinjé J
人类诺如病毒是全世界流行和地方性急性胃肠炎的主要原因，也是美国食源性疾病的主要原因。最近，从人的小肠组织中提取的人类肠类肠道(HIE)被证明支持人类诺如病毒的复制。我们在实验室实施了HIE系统，并测试了氯和酒精对人诺如病毒感染性的影响。观察到6种诺如病毒GII基因的复制成功，并且依赖于病毒载量和接种物的基因。GII-4病毒的复制水平高于其他基因型。无论浓度或暴露时间，酒精都略微减少了人类诺沃克病毒的活性，但并没有完全灭活。相反，当氯的浓度低至50ppm时，3GII-4病毒就会完全灭活。综上所述，我们的数据证实了人类诺如病毒在HIE中的成功复制，以及它们作为研究诺如病毒灭活策略的工具的实用性。
Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.
登录
查看更多内容
影响因子：
48
作者：
Yin, Xiaolei;Farin, Henner F.;van Es, Johan H.;Clevers, Hans;Langer, Robert;Karp, Jeffrey M.
通讯作者：
Karp, Jeffrey M.
影响因子：
24.5
作者：
VanDussen KL;Marinshaw JM;Shaikh N;Miyoshi H;Moon C;Tarr PI;Ciorba MA;Stappenbeck TS
通讯作者：
Stappenbeck TS
影响因子：
4.4
作者：
Kingsley, David H.;Fay, Johnna P.;Van Doren, Jane M.
通讯作者：
Van Doren, Jane M.
影响因子：
2
作者：
Cannon, Jennifer L.;Papafragkou, Efstathia;Vinje, Jan
通讯作者：
Vinje, Jan
影响因子：
4.4
作者：
KESWICK, BH;SATTERWHITE, TK;HOFF, JC
通讯作者：
HOFF, JC