Intravital imaging of islet Ca(2+) dynamics reveals enhanced β cell connectivity after bariatric surgery in mice.
Intravital imaging of islet Ca(2+) dynamics reveals enhanced β cell connectivity after bariatric surgery in mice.
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DOI：
10.1038/s41467-021-25423-8
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发表时间：
2021-08-27
影响因子：
16.6
通讯作者：
Rutter GA
中科院分区：
文献类型：
--
作者：
Akalestou E;Suba K;Lopez-Noriega L;Georgiadou E;Chabosseau P;Gallie A;Wretlind A;Legido-Quigley C;Leclerc I;Salem V;Rutter GA
减肥手术改善胰岛素敏感性和分泌，并可诱导糖尿病缓解。然而，这些变化的机制和时间过程，特别是对β细胞功能的影响，难以直接监测。在这项研究中，我们研究了垂直袖状胃切除术（VSG）对β细胞功能的影响，在体内通过成像钙离子动力学移植到眼前房的胰岛。由于其临床效用，VSG在小鼠中导致显著改善的葡萄糖耐量和增强的胰岛素分泌。我们发现，这些益处是由增强的β细胞功能和整个胰岛的协调活动所支撑的。这些效应涉及循环GLP-1水平的变化，其可能直接和间接作用于β细胞，在后一种情况下通过体重变化。因此，减肥手术导致β细胞功能和胰岛内连接的时间依赖性增加，这可能有助于糖尿病缓解。减肥手术程序减轻体重和改善代谢疾病。在本文中，作者使用体内Ca 2+成像研究了垂直袖状胃切除术（VSG）对小鼠β细胞功能的影响，并表明VSG增加了β细胞功能和胰岛内连接。
Bariatric surgery improves both insulin sensitivity and secretion and can induce diabetes remission. However, the mechanisms and time courses of these changes, particularly the impact on β cell function, are difficult to monitor directly. In this study, we investigated the effect of Vertical Sleeve Gastrectomy (VSG) on β cell function in vivo by imaging Ca2+ dynamics in islets engrafted into the anterior eye chamber. Mirroring its clinical utility, VSG in mice results in significantly improved glucose tolerance, and enhanced insulin secretion. We reveal that these benefits are underpinned by augmented β cell function and coordinated activity across the islet. These effects involve changes in circulating GLP-1 levels which may act both directly and indirectly on the β cell, in the latter case through changes in body weight. Thus, bariatric surgery leads to time-dependent increases in β cell function and intra-islet connectivity which are likely to contribute to diabetes remission. Bariatric surgery procedures reduce weight and improve metabolic diseases. Here the authors investigate the effects of Vertical Sleeve Gastrectomy (VSG) on beta-cell function in mice using in vivo Ca2 + imaging and show that VSG increases beta-cell function and intra-islet connectivity.
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仅在发生大量体重减轻并与减肥幅度相关后，才能观察到胃旁路手术后的外周葡萄糖吸收的改善。
影响因子：
3.2
作者：
Campos, Guilherme M.;Rabl, Charlotte;Peeva, Sofia;Ciovica, Ruxandra;Rao, Madhu;Schwarz, Jean-Marc;Havel, Peter;Schambelan, Morris;Mulligan, Kathleen
通讯作者：
Mulligan, Kathleen
影响因子：
2.9
作者：
Chambers, Adam P.;Stefater, Margaret A.;Wilson-Perez, Hilary E.;Jessen, Lene;Sisley, Stephanie;Ryan, Karen K.;Gaitonde, Shrawan;Sorrell, Joyce E.;Toure, Mouhamadoul;Berger, Jose;D'Alessio, David A.;Sandoval, Darleen A.;Seeley, Randy J.;Woods, Stephen C.
通讯作者：
Woods, Stephen C.
影响因子：
10.9
作者：
Benninger, Richard K. P.;Piston, David W.
通讯作者：
Piston, David W.
影响因子：
4.1
作者：
Ahonen, Linda;Jantti, Sirkku;Hyotylainen, Tuulia
通讯作者：
Hyotylainen, Tuulia
影响因子：
8.8
作者：
Garibay D;Lou J;Lee SA;Zaborska KE;Weissman MH;Sloma E;Donahue L;Miller AD;White AC;Michael MD;Sloop KW;Cummings BP
通讯作者：
Cummings BP