Evidence for preservation of vacuolar compartments during foehn-induced chalky ring formation of Oryza sativa L.
Evidence for preservation of vacuolar compartments during foehn-induced chalky ring formation of Oryza sativa L.
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DOI：
10.1007/s00425-018-2975-x
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发表时间：
2018-11
期刊：
影响因子：
4.3
通讯作者：
Wada H
中科院分区：
文献类型：
--
作者：
Hatakeyama Y;Masumoto-Kubo C;Nonami H;Morita S;Hiraoka K;Onda Y;Nakashima T;Nakano H;Wada H
水稻胚乳细胞内淀粉体积累不足，淀粉粒间存在的小室是干燥风条件下形成垩白圈的主要原因。在水稻灌浆期，焚风引起的干热风使水稻地上部水分亏缺。水稻胚乳中形成环状垩白，降低碾磨品质和稻米外观。由于不规则的光反射，在几个内部单元中形成的空气空间导致显著的透明度损失。虽然淀粉的合成被认为是在焚风引起的适度低水势的渗透调节延迟，这些空气空间的来源仍然未知。我们推测，液泡的保存伴随着内细胞中淀粉生物合成的暂时减少导致了白垩环的形成。进行穗水分状况测量、光学和透射电子显微镜（TEM）观察以及绝对qPCR分析。大多数淀粉合成相关基因表现出暂时减少表达的内区根据穗水分状况的下降。透射电镜观察表明，胚乳细胞内部的淀粉粒之间仍存在着液泡室，在胚乳细胞内部，淀粉粒脱水后出现了一个垩白圈。综上所述，我们提出，液泡室持续之间的淀粉体不充分积累在水稻胚乳细胞，并导致空气空间的形成，导致在干燥的风条件下的环形垩白。本文的在线版本（10.1007/s 00425 -018-2975-x）包含补充材料，可供授权用户使用。
Vacuolar compartments being sustained among the amyloplasts inadequately accumulated in rice endosperm cells are the main cause of chalky ring formation under dry wind conditions. Foehn-induced dry wind during the grain-filling stage induces shoot water deficit in rice (Oryza sativa L.) plants, which form a ring-shaped chalkiness in their endosperm that degrades milling quality and rice appearance. Air spaces formed in several inner cells cause significant transparency loss due to irregular light reflection. Although starch synthesis was suggested to be retarded by osmotic adjustment at foehn-induced moderately low water potential, the source of these air spaces remains unknown. We hypothesised that the preservation of vacuoles accompanied by a temporary reduction in starch biosynthesis in the inner cells leads to the chalky ring formation. Panicle water status measurement, light and transmission electron microscopic (TEM) observations, and an absolute qPCR analysis were conducted. Most starch synthesis-related genes exhibited temporarily reduced expression in the inner zone in accordance with the decrease in panicle water status. TEM observations provided evidence that vacuolar compartments remained among the loosely packed starch granules in the inner endosperm cells, where a chalky ring appeared after kernel dehydration. Taken together, we propose that vacuolar compartments sustained among the amyloplasts inadequately accumulated in rice endosperm cells and caused air space formation that leads to ring-shaped chalkiness under dry wind conditions. The online version of this article (10.1007/s00425-018-2975-x) contains supplementary material, which is available to authorized users.
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影响因子：
11.6
作者：
Marty, F
通讯作者：
Marty, F
影响因子：
11.6
作者：
Nakazono, M;Qiu, F;Schnable, PS
通讯作者：
Schnable, PS
影响因子：
2.3
作者：
Dou, Zhi;Tang, She;Ding, Yanfeng
通讯作者：
Ding, Yanfeng
影响因子：
7.4
作者：
Nakashima, Taiken;Wada, Hiroshi;Nonami, Hiroshi
通讯作者：
Nonami, Hiroshi
DOI：
10.1186/s12284-016-0100-y
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Kaneko K;Sasaki M;Kuribayashi N;Suzuki H;Sasuga Y;Shiraya T;Inomata T;Itoh K;Baslam M;Mitsui T
通讯作者：
Mitsui T