Transgenerational changes in the genome stability and methylation in pathogen-infected plants: (virus-induced plant genome instability).
Transgenerational changes in the genome stability and methylation in pathogen-infected plants: (virus-induced plant genome instability).
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DOI：
10.1093/nar/gkm029
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发表时间：
2007
影响因子：
14.9
通讯作者：
Kovalchuk I
中科院分区：
文献类型：
--
作者：
Boyko A;Kathiria P;Zemp FJ;Yao Y;Pogribny I;Kovalchuk I
以前，我们报道了一种病毒诱导的系统信号的产生，该信号增加了烟草花叶病毒（TMV）感染的烟草植株的体细胞和减数分裂重组率。在此，我们分析了接收到该信号的植物的后代，发现这些植物在携带抗TMV基因（n基因）LRR区同源基因的位点上也有更高的重排频率。对烟草基因座和5.8S核糖体RNA基因座重复元件的稳定性分析未见明显变化。对受感染植物后代变化的进一步分析表明，它们的基因组基本上是高甲基化的。与此同时，位点特异性甲基化分析显示：(1)多个lrr基因座存在深度低甲基化；(2)肌动蛋白位点大量高甲基化，(3)烟草N. tabacum或5.8S核糖体RNA重复元件位点甲基化无变化。后代的整体基因组高甲基化被认为是抗应激的一般保护机制的一部分，而位点特异性低甲基化则与更高频率的重排有关。重组事件的增加与病原体攻击引起的特定甲基化模式的结合可能是植物适应性反应的标志。
Previously, we reported the generation of a virus-induced systemic signal that increased the somatic and meiotic recombination rates in tobacco mosaic virus (TMV)-infected tobacco plants. Here, we analyzed the progeny of plants that received the signal and found that these plants also have a higher frequency of rearrangements in the loci carrying the homology to LRR region of the gene of resistance to TMV (N-gene). Analysis of the stability of repetitive elements from Nicotiana tabacum loci and 5.8S ribosomal RNA loci did not show any changes. Further analysis of the changes in the progeny of infected plants revealed that they had substantially hypermethylated genomes. At the same time, loci-specific methylation analysis showed: (1) profound hypomethylation in several LRR-containing loci; (2) substantial hypermethylation of actin loci and (3) no change in methylation in the loci of repetitive elements from N. tabacum or 5.8S ribosomal RNA. Global genome hypermethylation of the progeny is believed to be part of a general protection mechanism against stress, whereas locus-specific hypomethylation is associated with a higher frequency of rearrangements. Increased recombination events combined with the specific methylation pattern induced by pathogen attack could be a sign of an adaptive response by plants.
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影响因子：
7.2
作者：
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通讯作者：
Loake, GJ
影响因子：
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作者：
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RASCHKE, E
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Karpinski, S;Reynolds, H;Mullineaux, P
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