Expression analysis of matrix metalloproteinase-9 in epithelialized and nonepithelialized apical periodontitis lesions.
Expression analysis of matrix metalloproteinase-9 in epithelialized and nonepithelialized apical periodontitis lesions.
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DOI：
10.1016/j.tripleo.2008.07.030
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Granjeiro JM
中科院分区：
文献类型：
--
作者：
Carneiro E;Menezes R;Garlet GP;Garcia RB;Bramante CM;Figueira R;Sogayar M;Granjeiro JM
目的：探讨基质金属蛋白酶-9 （MMP-9）在根尖牙周炎病变中的表达。在根尖周手术后收集了19例上皮化和18例非上皮化的根尖牙周炎病变。经组织学处理、连续切片、H&E染色和显微镜分析，选择10个上皮化和10个非上皮化病变进行MMP-9和cd68的免疫组化分析。每个标本至少1/3在- 70°C冷冻，进一步分离mRNA并反转录成cDNA进行Real-Time-PCR程序。目的基因的相对表达量与内参基因（GAPDH、HPRT、β-actin和BCRP）比较。在两种类型的病变中，对多形核中性粒细胞、巨噬细胞和淋巴细胞进行MMP-9染色，当存在时，也对上皮细胞进行染色。非上皮化病变的MMP-9+/总细胞数和比例大于上皮化病变（p=0.0001），与CD68+/总细胞数呈正相关（p=0.045）。MMP-9 mRNA的表达在上皮病变和非上皮病变之间无显著差异。然而，与健康的根尖周围韧带相比，两种病变类型的MMP-9表达均增加（p<0.0001）。目前的数据表明，几种炎症细胞（主要是CD68+细胞）参与了根尖牙周炎病变中MMP-9的表达。MMP-9可能积极参与根尖牙周炎病变中ECM的降解。
To determine the expression of matrix metalloproteinase-9 (MMP-9) in apical periodontitis lesions. Nineteen epithelialized and eighteen non-epithelialized apical periodontitis lesions were collected after periapical surgery. After histological processing, serial sectioning, H&E staining and microscopic analysis, 10 epithelialized and 10 non-epithelialized lesions were selected for immunohistochemical analysis for MMP-9 and CD 68. At least 1/3 of each specimen was frozen at −70°C for further mRNA isolation and reverse transcription into cDNA for Real-Time-PCR procedures. The relative expression of a target gene was determined in comparison with reference genes (GAPDH, HPRT, β-actin and BCRP). Polymorphonuclear neutrophils, macrophages and lymphocytes were stained for MMP-9 in both types of lesions, and when present, epithelial cells were also stained. The number and the ratio of MMP-9+/total cells were greater in non-epithelialized than epithelialized lesions (p=0.0001) and showed a positive correlation to CD68+/total cells (p=0.045). No significant differences were observed for MMP-9 mRNA expression between ephithelized and non-ephithelized lesions. However, when compared to healthy periapical ligaments, both types of lesions presented increased MMP-9 expression (p<0.0001). The present data suggest the participation of several inflammatory cells, mainlly CD68+ cells, in the MMP-9 expression in apical periodontitis lesions. MMP-9 could be actively enroled in the ECM degradation in apical periodontitis lesions.
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影响因子：
1.9
作者：
TERONEN, O;SALO, T;SORSA, T
通讯作者：
SORSA, T
DOI：
10.1016/j.tripleo.2005.10.054
发表时间：
2006-09-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Menezes, Renato;Bramante, Clovis Monteiro;Granjeiro, Jose Mauro
通讯作者：
Granjeiro, Jose Mauro
影响因子：
5
作者：
Leonardi, R;Caltabiano, R;Loreto, C
通讯作者：
Loreto, C
DOI：
10.1016/s1079-2104(96)80156-9
发表时间：
1996-01-01
影响因子：
--
作者：
Ramachandran, PN;Pajorola, G;Schroeder, HE
通讯作者：
Schroeder, HE
影响因子：
--
作者：
Silva, TA;Garlet, GP;Cunha, FQ
通讯作者：
Cunha, FQ