Cell synchronization for the purposes of nuclear transfer in the bovine.
Cell synchronization for the purposes of nuclear transfer in the bovine.
复制标题
用于牛核移植目的的细胞同步。
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
O. Lacham‐Kaplan
中科院分区：
文献类型：
--
作者：
N. Korfiatis;A. Trounson;O. Lacham‐Kaplan
我们使用牛核移植（NT）模型检测了供体成纤维细胞核在细胞周期的不同阶段转移到细胞质后的重编程能力。牛胎成纤维细胞在还原血清和条件培养基中培养以诱导静止（G0），并用诺可达唑诱导M期停止。非同步活跃分裂细胞（对照组）主要在G1期。将同步于G0期、M期和G1期的细胞直接注射到去核的牛MII卵母细胞中。注射后人工激活NT卵母细胞。M期细胞经人工活化后也转移到去核卵母细胞中。血清饥饿诱导休眠的细胞和未同步供体细胞发育至桑葚胚/囊胚期的比例最高（分别为20%和18%）。孤雌生殖对照的囊胚发育明显高于NT胚胎。M期细胞核向MII细胞质的转移与囊胚期的高度发育无关。然而，确定这些胚胎的生存能力需要移植到受体动物和评估体内发育。
We have examined the reprogramming ability of donor fibroblast nuclei in various phases of the cell cycle, upon transfer to cytoplasts, using a bovine nuclear transfer (NT) model. Bovine fetal fibroblasts were cultured in reduced serum and conditioned medium to induce quiescence (G0) and treated with nocodazole to induce M phase arrest. Unsynchronized actively dividing cells (control) were mainly in G1. Cells synchronized in G0, M, and G1 phase were transferred to enucleated bovine MII oocytes by direct injection using the Piezo-Drill microinjector. NT oocytes were artificially activated following injection. Cells at the M phase were also transferred to enucleated oocytes after artificial activation. Cells induced into quiescence by serum starvation and unsynchronized donor cells produced the highest rates of development to the morula/blastocyst stage (20% and 18%, respectively). Development to blastocyst was significantly higher in parthenogenetic controls compared to NT embryos. The transfer of M phase nuclei to MII cytoplasts was not associated with high development to the blastocyst stage. Nevertheless, determining the viability of these embryos requires transfer to recipient animals and assessment of in vivo development.
登录
查看更多内容
影响因子：
3.6
作者：
KIMURA, Y;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R
影响因子：
3.6
作者：
Boquest, AC;Day, BN;Prather, RS
通讯作者：
Prather, RS
影响因子：
56.9
作者：
HARTWELL, LH;WEINERT, TA
通讯作者：
WEINERT, TA
影响因子：
56.9
作者：
Lanza, RP;Cibelli, JB;West, MD
通讯作者：
West, MD