Demonstration of strain-specific CD8 T cell responses to Theileria annulata.
Demonstration of strain-specific CD8 T cell responses to Theileria annulata.
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DOI：
10.1111/j.1365-3024.2008.01038.x
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发表时间：
2008-08
影响因子：
2.2
通讯作者：
Morrison WI
中科院分区：
文献类型：
--
作者：
Machugh ND;Burrells AC;Morrison WI
本研究旨在研究牛CD8 T细胞反应的性质和特异性，在克隆水平上，在一组8只动物免疫的克隆群体的环形泰勒虫。结果表明，免疫动物产生寄生虫特异性CD8 T细胞，其响应寄生虫刺激产生IFNγ，但对寄生细胞具有高度可变的细胞毒性水平。研究还表明，这些寄生虫特异性的CD8 T细胞可以在体外从用活T.环纹寄生虫在研究的一小组动物中，有证据表明，反应优先针对A10+ I类主要组织相容性复合体（MHC）单倍型呈递的抗原，这表明该单倍型产物限制的反应可能是显性的。A10限制性反应显示不同寄生虫分离株和克隆的差异识别。通过使用克隆的寄生虫种群的免疫接种的动物和在体外分析的反应，我们获得了明确的证据，至少有一部分的CD8 T细胞限制的一个MHC单倍型的寄生虫株限制。
The present study set out to examine the nature and specificity of the bovine CD8 T cell response at the clonal level in a group of eight animals immunized with a cloned population of Theileria annulata. The results demonstrated that immunized animals generated parasite-specific CD8 T cells that produced IFNγ in response to parasite stimulation but had highly variable levels of cytotoxicity for parasitized cells. The study also demonstrated that these parasite-specific CD8 T cells could be propagated and cloned in vitro from the memory T cell pool of cattle immunized with live T. annulata parasites. Within the small group of animals studied, there was evidence that responses were preferentially directed to antigens presented by an A10+ class I major histocompatibility complex (MHC) haplotype, suggesting that responses restricted by products of this haplotype may be dominant. The A10-restricted responses showed differential recognition of different parasite isolates and clones. By using a cloned population of parasites both for immunization of the animals and for in vitro analyses of the responses, we obtained unambiguous evidence that at least a proportion of CD8 T cells restricted by one MHC haplotype were parasite strain restricted.
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影响因子：
64.8
作者：
BROWN, CGD;STAGG, DA;PAYNE, RC
通讯作者：
PAYNE, RC
影响因子：
56.9
作者：
Pain, A;Renauld, H;Hall, N
通讯作者：
Hall, N
影响因子：
3.2
作者：
Ellis, SA;Holmes, EC;Morrison, WI
通讯作者：
Morrison, WI
影响因子：
1.8
作者：
HOWARD, CJ;SOPP, P;BROWN, CGD
通讯作者：
BROWN, CGD
DOI：
10.1111/j.1749-6632.2000.tb05328.x
发表时间：
2000-01-01
期刊：
TROPICAL VETERINARY DISEASES
影响因子：
--
作者：
Pipano, E;Shkap, V
通讯作者：
Shkap, V