Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh.
Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh.
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Ca2交联海藻酸盐水凝胶可促进脊髓损伤后脊髓神经干/祖细胞分化和功能恢复hh。
DOI：
10.1093/rb/rbac057
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发表时间：
2022
影响因子：
6.7
通讯作者：
Liu, Shengwen
中科院分区：
文献类型：
--
作者：
Zhou, Jun;Wu, Yaqi;Tang, Zhijian;Zou, Kaipeng;Chen, Juan;Lei, Zuowei;Wan, Xueyan;Liu, Yanchao;Zhang, Huaqiu;Wang, Yu;Blesch, Armin;Lei, Ting;Liu, Shengwen
海藻酸盐毛细血管水凝胶植入分化细胞后，可填充损伤腔，促进损伤脊髓轴突再生。神经干细胞/祖细胞（NSPCs）可以潜在地修复脊髓；然而，藻酸盐水凝胶（AHs）对NSPCs的影响尚不清楚。在这项研究中，我们用Ca2+交联的AHs和种子水凝胶制备了大鼠胚胎第14天的NSPCs。免疫细胞化学和电镜显示，NSPCs在体外毛细血管内存活、增殖并分化为神经元。移植到成年大鼠急性T8完全性脊髓横断部位后，约三分之一（38.3%）的移植细胞存活，并在移植8周后分化为神经元（40.7%）、星形胶质细胞（26.6%）和少突胶质细胞（28.4%）。NSPCs以一种时间依赖性的方式促进毛细血管内宿主轴突的生长。宿主轴突在水凝胶通道内与nspc来源的神经元进行突触样接触，移植物来源的轴突延伸到宿主白质和灰质中形成假定的突触。这与病变部位的电生理传导改善和部分后肢运动恢复是平行的。
Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can potentially repair the spinal cord; however, effects of alginate hydrogels (AHs) on NSPCs remain unknown. In this study, we fabricated AHs cross-linked by Ca2+ and seeded hydrogels with rat embryonic day 14 NSPCs. Immunocytochemistry and electron microscopy show that NSPCs survive, proliferate and differentiate into neurons in vitro within the capillaries. After transplantation into an acute T8 complete spinal cord transection site in adult rats, approximately one-third (38.3%) of grafted cells survive and differentiate into neurons (40.7%), astrocytes (26.6%) and oligodendrocytes (28.4%) at 8 weeks post-grafting. NSPCs promote the growth of host axons within the capillaries in a time-dependent manner. Host axons make synapse-like contacts with NSPC-derived neurons within the hydrogel channels, and graft-derived axons extend into the host white and gray matter making putative synapses. This is paralleled by improved electrophysiological conductivity across the lesion and partial hindlimb locomotor recovery.
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影响因子：
16.2
作者：
MayerProschel, M;Kalyani, AJ;Rao, MS
通讯作者：
Rao, MS
影响因子：
1.2
作者：
Lu, Paul;Graham, Lori;Tuszynski, Mark
通讯作者：
Tuszynski, Mark
影响因子：
15.9
作者：
Lu, Paul;Ceto, Steven;Tuszynski, Mark H.
通讯作者：
Tuszynski, Mark H.
影响因子：
5.8
作者：
Ansorena, Eduardo;De Berdt, Pauline;des Rieux, Anne
通讯作者：
des Rieux, Anne
影响因子：
4.6
作者：
Grulova I;Slovinska L;Blaško J;Devaux S;Wisztorski M;Salzet M;Fournier I;Kryukov O;Cohen S;Cizkova D
通讯作者：
Cizkova D