Insights into the evolution of sialic acid catabolism among bacteria.
Insights into the evolution of sialic acid catabolism among bacteria.
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DOI：
10.1186/1471-2148-9-118
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发表时间：
2009-05-26
影响因子：
3.4
通讯作者：
Boyd EF
中科院分区：
文献类型：
--
作者：
Almagro-Moreno S;Boyd EF
唾液酸由九碳氨基糖组成，普遍存在于粘液丰富的环境中。来自人类宿主的唾液酸被许多病原体用作能量来源。在这里，我们探索参与唾液酸分解代谢的基因的进化。编码唾液酸分解代谢所必需的 N-乙酰神经氨酸裂解酶 (NanA)、差向异构酶 (NanE) 和激酶 (NanK) 的基因簇（Nan 簇）局限于 46 种细菌，其中 42 种定植于哺乳动物，33 种为病原体，9 种为肠道共生菌。我们在大多数物种中发现了与 Nan 簇相关的假定唾液酸转运蛋白。我们重建了 46 个物种的 NanA、NanE 和 NanK 蛋白的系统发育历史，并将它们与基于 16S rRNA 的物种树进行了比较。在 NanA 系统发育中，革兰氏阴性菌和革兰氏阳性菌不形成不同的进化枝。耶尔森氏菌和弧菌属的 NanA 与真核生物的 NanA 进化枝关系最为密切。为了进一步研究这一点，我们重建了数据库中所有 NanA 同源物的系统发育。在对 83 个 NanA 序列的分析中，人类共生拟杆菌门与疣微菌门形成了一个独特的分支，并与真核生物和耶尔森氏菌/弧菌分支形成了分支。我们推测霍乱弧菌等病原体可能从共生体中获得了 NanA，从而帮助它们在人类肠道中定殖。 NanE 和 NanK 系统发育更接近地代表了物种树，但也注意到了许多不一致的情况。我们证实了主要肠道病原体肠沙门氏菌、霍乱弧菌、创伤弧菌、小肠结肠炎耶尔森菌和鼠疫耶尔森菌成员中唾液酸分解代谢簇的预测功能。细菌中的 Nan 簇仅限于人类病原体和共生体，使它们能够利用人体富含粘液的表面中普遍存在的碳源。 Nan区域显示出嵌合进化，来自拟杆菌、弧菌和耶尔森氏菌的NanA与来自真核生物的NanA紧密分支。
Sialic acids comprise a family of nine-carbon amino sugars that are prevalent in mucus rich environments. Sialic acids from the human host are used by a number of pathogens as an energy source. Here we explore the evolution of the genes involved in the catabolism of sialic acid. The cluster of genes encoding the enzymes N-acetylneuraminate lyase (NanA), epimerase (NanE), and kinase (NanK), necessary for the catabolism of sialic acid (the Nan cluster), are confined 46 bacterial species, 42 of which colonize mammals, 33 as pathogens and 9 as gut commensals. We found a putative sialic acid transporter associated with the Nan cluster in most species. We reconstructed the phylogenetic history of the NanA, NanE, and NanK proteins from the 46 species and compared them to the species tree based on 16S rRNA. Within the NanA phylogeny, Gram-negative and Gram-positive bacteria do not form distinct clades. NanA from Yersinia and Vibrio species was most closely related to the NanA clade from eukaryotes. To examine this further, we reconstructed the phylogeny of all NanA homologues in the databases. In this analysis of 83 NanA sequences, Bacteroidetes, a human commensal group formed a distinct clade with Verrucomicrobia, and branched with the Eukaryotes and the Yersinia/Vibrio clades. We speculate that pathogens such as V. cholerae may have acquired NanA from a commensal aiding their colonization of the human gut. Both the NanE and NanK phylogenies more closely represented the species tree but numerous incidences of incongruence are noted. We confirmed the predicted function of the sialic acid catabolism cluster in members the major intestinal pathogens Salmonella enterica, Vibrio cholerae, V. vulnificus, Yersinia enterocolitica and Y. pestis. The Nan cluster among bacteria is confined to human pathogens and commensals conferring them the ability to utilize a ubiquitous carbon source in mucus rich surfaces of the human body. The Nan region shows a mosaic evolution with NanA from Bacteroidetes, Vibrio and Yersinia branching closely together with NanA from eukaryotes.
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影响因子：
3.1
作者：
Allen, S;Zaleski, A;Apicella, MA
通讯作者：
Apicella, MA
影响因子：
3
作者：
Malykh, YN;Krisch, B;Schauer, R
通讯作者：
Schauer, R
影响因子：
13.8
作者：
Dandekar, T;Snel, B;Bork, P
通讯作者：
Bork, P
影响因子：
3.2
作者：
Brigham, Christopher;Caughlan, Ruth;Malamy, Michael H.
通讯作者：
Malamy, Michael H.
影响因子：
4.2
作者：
Lee KC;Webb RI;Janssen PH;Sangwan P;Romeo T;Staley JT;Fuerst JA
通讯作者：
Fuerst JA