ALS1 Deletion Increases the Proportion of Small Cells in a Candida albicans Culture Population: Hypothesizing a Novel Role for Als1.
ALS1 Deletion Increases the Proportion of Small Cells in a Candida albicans Culture Population: Hypothesizing a Novel Role for Als1.
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ALS1缺失增加了白色念珠菌培养群体中小细胞的比例：假设ALS1的新作用。
DOI：
10.3389/fcimb.2022.895068
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发表时间：
2022
影响因子：
5.7
通讯作者：
Hoyer, Lois L.
中科院分区：
文献类型：
--
作者：
Zhao, Xiaomin;Oh, Soon-Hwan;Coleman, David A.;Hoyer, Lois L.
关键词：
白色念珠菌Als 1是一种大的细胞表面糖蛋白，最常讨论的是其在介导配体结合和聚集相互作用中的作用。相对于野生型对照，ALS 1的缺失产生了一种菌株，该菌株在播散性念珠菌病的小鼠模型中显示出延迟的生殖管形成和延迟的疾病进展。与野生型或ALS 1重组对照培养物相比，Δ als 1/Δ als 1培养细胞群体中较小细胞的比例更高。这项工作的目的是研究这种细胞大小分布的差异是否是延迟生殖管形成和延迟疾病进展的原因。使用流式细胞术选择具有不同细胞大小分布的野生型和Δ als 1/Δ als 1细胞群。从野生型（ALS 1/ALS 1）培养群体中分选的小细胞证明了延迟的胚管形成。从Δ als 1/Δ als 1培养物中分选的大细胞与野生型对照一样快地形成芽管，这清楚地表明Δ als 1/Δ als 1芽管形成延迟可归因于细胞大小。在体内，野生型对照的较小尺寸的细胞与相同菌株的较大细胞相比，每克肾组织的集落形成单位（cfu）较少，组织病理学病变不太严重。与野生型对照组相比，Δ als 1/Δ als 1菌株显示肾组织cfu/g减少，病变不太严重。然而，从Δ als 1/Δ als 1群体中分离和检测较大细胞增加了cfu/g组织，并显示与总体突变细胞群体相比病变严重程度增加。大型分选的Δ als 1/Δ als 1细胞的体内菌丝长度与野生型对照菌株的菌丝长度相当。这些结果表明，大部分Δ als 1/Δ als 1体内表型可归因于细胞大小。总的来说，这些数据表明Als 1在C.白念珠菌细胞大小稳态，一个新的假说，进一步探索。
Candida albicans Als1 is a large cell-surface glycoprotein most often discussed for its role in mediating ligand-binding and aggregative interactions. Relative to a wild-type control, deletion of ALS1 produced a strain that showed delayed germ-tube formation and delayed disease progression in a murine model of disseminated candidiasis. Populations of Δals1/Δals1 cultured cells had a higher proportion of smaller cells compared to wild-type or ALS1 reintegrant control cultures. The goal of this work was to investigate whether this difference in cell-size distributions was responsible for delayed germ-tube formation and delayed disease progression. Flow cytometry was used to select populations of wild-type and Δals1/Δals1 cells with varied cell-size distributions. Delayed germ-tube formation was demonstrated for small cells sorted from a wild-type (ALS1/ALS1) culture population. Large cells sorted from a Δals1/Δals1 culture formed germ tubes as quickly as the wild-type control demonstrating clearly that the Δals1/Δals1 germ-tube formation delays were attributable to cell size. In vivo, smaller-sized cells of the wild-type control showed fewer colony-forming units (cfu) per gram of kidney tissue and less-severe histopathology lesions compared to larger cells of the same strain. The Δals1/Δals1 strain showed reduced cfu/g of kidney tissue and less-severe lesions compared to the wild-type control. However, isolation and testing of the larger cells from the Δals1/Δals1 population increased cfu/g of tissue and showed increased lesion severity compared to the overall mutant cell population. In vivo hypha lengths from the large, sorted Δals1/Δals1 cells were comparable to those for the wild-type control strain. These results demonstrated that a large share of the Δals1/Δals1 in-vivo phenotype was attributable to cell size. Collectively, the data suggest a role for Als1 in C. albicans cell size homeostasis, a novel hypothesis for further exploration.
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影响因子：
5.2
作者：
Hoyer LL;Cota E
通讯作者：
Cota E
影响因子：
3.7
作者：
SOLL, DR;BEDELL, G;BRUMMEL, M
通讯作者：
BRUMMEL, M
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
3.2
作者：
Porta, A;Ramon, AM;Fonzi, WA
通讯作者：
Fonzi, WA
DOI：
10.1083/jcb.75.2.422
发表时间：
1977-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hartwell LH;Unger MW
通讯作者：
Unger MW