Multimodal nonlinear optical imaging of collagen arrays.
Multimodal nonlinear optical imaging of collagen arrays.
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DOI：
10.1016/j.jsb.2008.07.002
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发表时间：
2008-10
影响因子：
3
通讯作者：
Legare, Francois
中科院分区：
文献类型：
--
作者：
Pfeffer, Christian P.;Olsen, Bjorn R.;Ganikhanov, Feruz;Legare, Francois
我们报告了筋膜的多模态非线性光学成像，筋膜是内脏和肌肉周围富含 I 型胶原蛋白的鞘。我们表明，二次谐波发生（SHG）、三次谐波发生（THG）和相干反斯托克斯拉曼散射（CARS）显微镜技术提供了有关胶原蛋白阵列亚微米结构的补充信息。正向 SHG 显微镜揭示了 I 型胶原蛋白结构的纤维排列是筋膜的主要基质成分。后向SHG图像以及前向CARS显微镜图像显示，纵向胶原纤维束进一步排列成片状带状。正向 THG 显微镜揭示了胶原片在光学波长空间尺度上的光学均匀含量。仅在板材之间的边界处观察到三次谐波信号以及在两个方向上获得的 CARS 数据这一事实支持了这一点。使用原子力显微镜图像解释了使用 THG 和 CARS 显微镜进行的观察。
We report multimodal nonlinear optical imaging of fascia, a rich collagen type-I sheath around internal organs and muscle. We show that second harmonic generation (SHG), third harmonic generation (THG) and Coherent anti-Stokes Raman scattering (CARS) microscopy techniques provide complementary information about the sub-micron architecture of collagen arrays. Forward direction SHG microscopy reveals the fibrillar arrangement of collagen Type I structures as the main matrix component of fascia. SHG images detected in the backward direction as well as images of forward direction CARS microscopy show that the longitudinal collagen fiber bundles are further arranged in sheet like bands. Forward THG microscopy reveals the optically homogeneous content of the collagen sheet on a spatial scale of the optical wavelength. This is supported by the fact that the third harmonic signal is observed only at the boundaries between the sheets as well as by the CARS data obtained in both directions. The observations made with THG and CARS microscopy are explained using Atomic Force Microscopy images.
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