Knockout of the VPS22 component of the ESCRT-II complex in rice (Oryza sativa L.) causes chalky endosperm and early seedling lethality
Knockout of the VPS22 component of the ESCRT-II complex in rice (Oryza sativa L.) causes chalky endosperm and early seedling lethality
复制标题
水稻 (Oryza sativa L.) 中 ESCRT-II 复合物的 VPS22 成分的敲除会导致白垩胚乳和早期幼苗致死
DOI：
10.1007/s11033-012-2422-1
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发表时间：
2013-05
影响因子：
2.8
通讯作者：
Xin-Ming Xie
中科院分区：
文献类型：
--
作者：
Xiang-Qian Zhang;Pei Hou;Hai-Tao Zhu;Guo-dong Li;Xin-Guo Liu;Xin-Ming Xie
在酵母和哺乳动物中，转运蛋白-II（ESCRT-II）所需的内体分选复合物的主要成分是VPS 22/EAP 30蛋白，其在泛素介导的膜蛋白降解中起重要作用。然而，ESCRT-II亚基在植物中的功能在很大程度上是未知的。本研究报道了水稻VPS 22基因OsVPS 22突变体的遗传分析和表型特征。在收集的T-DNA株系的基础上，我们鉴定了一个T-DNA插入突变体，该突变体表现出异常分离比，并发现T-DNA插入位于OsVPS 22基因的第6内含子内。与野生型相比，该vps 22突变体表现出幼苗致死性，地上部和根系生长严重下降。此外，vps 22突变体在籽粒中具有垩白胚乳。综上所述，我们的数据表明OsVPS 22可能是水稻幼苗存活和籽粒灌浆所必需的，从而为进一步探索植物中ESCR机制的功能提供了宝贵的资源。
In both yeast and mammals, the major constituent of the endosomal sorting complex required for transport-II (ESCRT-II) is the VPS22/EAP30 protein, which plays an important role in ubiquitin-mediated degradation of membrane proteins through the multivesicular body pathway. However, the functions of ESCRT-II subunits in plants are largely unknown. In this work, we report the genetic analysis and phenotypic characterization of mutants in OsVPS22 gene, which encodes a functional VPS22 homolog in rice. On the basis of a collection of T-DNA lines, we identified a T-DNA insertion mutant, which showed abnormal segregation ratios; we then found that the T-DNA insertion is located within the sixth intron of the OsVPS22 gene. Compared with the wild type, this vps22 mutant exhibited seedling lethality and severe reduction in shoot and root growth. In addition, the vps22 mutant had a chalky endosperm in the grain. In summary, our data suggest that OsVPS22 may be required for seedling viability and grain filling in rice, thus providing a valuable resource for further exploration of the functions of the ESCRTing machinery in plants.
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影响因子：
64.5
作者：
Katzmann, DJ;Babst, M;Emr, SD
通讯作者：
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