Rapid joule heating improves vitrification based cryopreservation.
Rapid joule heating improves vitrification based cryopreservation.
复制标题
DOI：
10.1038/s41467-022-33546-9
复制
发表时间：
2022-10-12
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
Cryopreservation by vitrification has far-reaching implications. However, rewarming techniques that are rapid and scalable (both in throughput and biosystem size) for low concentrations of cryoprotective agent (CPA) for reduced toxicity are lacking, limiting the potential for translation. Here, we introduce a joule heating–based platform technology, whereby biosystems are rapidly rewarmed by contact with an electrical conductor that is fed a voltage pulse. We demonstrate successful cryopreservation of three model biosystems with thicknesses across three orders of magnitude, including adherent cells (~4 µm), Drosophila melanogaster embryos (~50 µm) and rat kidney slices (~1.2 mm) using low CPA concentrations (2–4 M). Using tunable voltage pulse widths from 10 µs to 100 ms, numerical simulation predicts that warming rates from 5 × 104 to 6 × 108 °C/min can be achieved. Altogether, our results present a general solution to the cryopreservation of a broad spectrum of cellular, organismal and tissue-based biosystems. Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and demonstrate the preservation of Drosophila embryos and rat kidney slices.
登录
查看更多内容
影响因子：
3.2
作者：
Haemmerich, D;Staelin, ST;Webster, JG
通讯作者：
Webster, JG
影响因子：
4.1
作者：
Khosla K;Kangas J;Liu Y;Zhan L;Daly J;Hagedorn M;Bischof J
通讯作者：
Bischof J
影响因子：
--
作者：
Etheridge, Michael L.;Xu, Yi;Bischof, John C.
通讯作者：
Bischof, John C.
影响因子：
2.7
作者：
Bissoyi, Akalabya;Braslavsky, Ido
通讯作者：
Braslavsky, Ido
影响因子：
2.8
作者：
Fujino, Y.;Kojima, T.;Funahashi, H.
通讯作者：
Funahashi, H.