Metagenomic analysis of the Rhinopithecus bieti fecal microbiome reveals a broad diversity of bacterial and glycoside hydrolase profiles related to lignocellulose degradation.
Metagenomic analysis of the Rhinopithecus bieti fecal microbiome reveals a broad diversity of bacterial and glycoside hydrolase profiles related to lignocellulose degradation.
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金丝猴粪便微生物组的宏基因组分析揭示了与木质纤维素降解相关的细菌和糖苷水解酶谱的广泛多样性
DOI：
10.1186/s12864-015-1378-7
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发表时间：
2015-03-12
期刊：
影响因子：
4.4
通讯作者：
Huang Z
中科院分区：
文献类型：
--
作者：
Xu B;Xu W;Li J;Dai L;Xiong C;Tang X;Yang Y;Mu Y;Zhou J;Ding J;Wu Q;Huang Z
研究背景动物胃肠道含有复杂的微生物群落，其组成最终反映了微生物与其动物宿主以及宿主饮食的共同进化。尽管人类肠道微生物群的重要性已得到充分证明，但关于非人类灵长类动物 (NHP)，尤其是草食性 NHP 的研究却很少。结果在本研究中，对从金丝猴粪便 DNA 提取物中生成的 97,942 个焦磷酸测序读数进行了分析，以帮助更好地了解金丝猴肠道微生物组的微生物多样性和功能能力。宏基因组读数的分类分析表明，R.bieti 粪便微生物组以厚壁菌门、拟杆菌门、变形菌门和放线菌门为主。分类学分类的比较分析表明，与其他动物相比，R. bieti 的宏基因组的特点是纤维杆菌门和螺旋菌门的细菌过多。主要功能类别主要与蛋白质、碳水化合物、氨基酸、DNA 和 RNA 代谢、辅助因子、细胞壁和荚膜以及膜运输相关。将R. bieti与其他动物的糖苷水解酶谱进行比较，发现R. bieti微生物组与牛瘤胃的关系最为密切。结论这些结果将有助于有限的NHP宏基因组研究，并为植物细胞壁降解微生物酶提供独特的遗传资源。然而，未来需要对 R. bieti 的宏基因组测序进行研究，了解年龄、遗传、饮食和环境对宏基因组的组成和活性的影响。
BackgroundThe animal gastrointestinal tract contains a complex community of microbes, whose composition ultimately reflects the co-evolution of microorganisms with their animal host and the diet adopted by the host. Although the importance of gut microbiota of humans has been well demonstrated, there is a paucity of research regarding non-human primates (NHPs), especially herbivorous NHPs.ResultsIn this study, an analysis of 97,942 pyrosequencing reads generated from Rhinopithecus bieti fecal DNA extracts was performed to help better understanding of the microbial diversity and functional capacity of the R. bieti gut microbiome. The taxonomic analysis of the metagenomic reads indicated that R. bieti fecal microbiomes were dominated by Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria phyla. The comparative analysis of taxonomic classification revealed that the metagenome of R. bieti was characterized by an overrepresentation of bacteria of phylum Fibrobacteres and Spirochaetes as compared with other animals. Primary functional categories were associated mainly with protein, carbohydrates, amino acids, DNA and RNA metabolism, cofactors, cell wall and capsule and membrane transport. Comparing glycoside hydrolase profiles of R. bieti with those of other animal revealed that the R. bieti microbiome was most closely related to cow rumen.Conclusions These results would contribute to the limited body of NHPs metagenome studies and provide a unique genetic resource of plant cell wall degrading microbial enzymes. However, future studies on the metagenome sequencing of R. bieti regarding the effects of age, genetics, diet and environment on the composition and activity of the metagenomes are required.
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DOI：
10.1098/rspb.2008.0284
发表时间：
2008-07-22
影响因子：
4.7
作者：
Davies, T. Jonathan;Pedersen, Amy B.
通讯作者：
Pedersen, Amy B.
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
DOI：
10.1073/pnas.0806191105
发表时间：
2009-02-10
影响因子：
11.1
作者：
Brulc, Jennifer M.;Antonopoulos, Dionysios A.;White, Bryan A.
通讯作者：
White, Bryan A.
影响因子：
4.4
作者：
Hongoh, Yuichi;Deevong, Pinsurang;Ohkuma, Moriya
通讯作者：
Ohkuma, Moriya
影响因子：
2.8
作者：
Bhatt, Vaibhav D.;Dande, Suchitra S.;Joshi, Chaitanya G.
通讯作者：
Joshi, Chaitanya G.