A study of diffusion in poly(ethyleneglycol)-gelatin based semi-interpenetrating networks for use in wound healing.
A study of diffusion in poly(ethyleneglycol)-gelatin based semi-interpenetrating networks for use in wound healing.
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DOI：
10.1007/s00289-008-0023-x
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发表时间：
2009-03-01
期刊：
影响因子：
3.2
通讯作者：
Kao, W. John
中科院分区：
文献类型：
--
作者：
Bader, Rebecca Ann;Herzog, Kyle T.;Kao, W. John
半互穿网络（sIPN），旨在模拟细胞外基质通过共价交联的聚（乙二醇）二丙烯酸酯在明胶的存在下，已被证明有助于伤口愈合，特别是当负载可溶性因子。用于组织修复的理想系统允许治疗剂的有效释放和营养物向增殖细胞的流动。因此，适当的网络表征可以用于传达对材料所需的传质动力学的理解，以帮助伤口愈合过程。溶质运输和通过sIPN尚未得到彻底的评价。在本研究中，生长因子和营养物质通过聚合物系统的扩散率进行了测定。角质形成细胞生长因子的转运通过将sIPN处理为蛋白质加载到其中的平面片来建模。扩散系数为4.86 × 10−9 ± 1.86 × 10−12 cm 2/s。葡萄糖转运被建模为通过半透膜的流动。通过滞后时间分析，计算出扩散系数为2.25 × 10−6 ± 1.98 × 10−7 cm 2/s。结合以前的研究对药物从sIPN的控制释放的结果进行了评价。正如Einstein-Stokes方程所预期的那样，扩散系数随着分子尺寸的增加而降低。结果提供了深入了解的结构-功能设计范例，并表明，从聚合物系统的释放是扩散控制，而不是溶解控制。
Semi-interpenetrating networks (sIPNs) designed to mimic extracellular matrix via covalent crosslinking of poly(ethylene glycol) diacrylate in the presence of gelatin have been shown to aid in wound healing, particularly when loaded with soluble factors. Ideal systems for tissue repair permit an effective release of therapeutic agents and flow of nutrients to proliferating cells. Appropriate network characterization can, consequently, be used to convey an understanding of the mass transfer kinetics necessary for materials to aid in the wound healing process. Solute transport from and through sIPNs has not yet been thoroughly evaluated. In the current study, the diffusivity of growth factors and nutrients through the polymeric system was determined. Transport of keratinocyte growth factor was modeled by treating the sIPN as a plane sheet into which the protein was loaded. The diffusion coefficient was determined to be 4.86 × 10−9 ± 1.86 × 10−12 cm2/s. Glucose transport was modeled as flow through a semi-permeable membrane. Using lag-time analysis, the diffusion coefficient was calculated to be 2.25 × 10−6 ± 1.98 × 10−7 cm2/s. The results were evaluated in conjunction with previous studies on controlled drug release from sIPNs. As expected from Einstein-Stokes equation, diffusivity decreased as molecular size increased. The results offer insight into the structure-function design paradigm and show that release from the polymeric system is diffusion controlled, rather than dissolution controlled.
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