Engineered alginate hydrogels for effective microfluidic capture and release of endothelial progenitor cells from whole blood.
Engineered alginate hydrogels for effective microfluidic capture and release of endothelial progenitor cells from whole blood.
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DOI：
10.1021/la105016a
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发表时间：
2011-04-05
期刊：
影响因子：
--
通讯作者：
Murthy SK
中科院分区：
文献类型：
--
作者：
Hatch A;Hansmann G;Murthy SK
基于微流体黏附的细胞分离系统在临床和生物应用中备受关注，在这些应用中必须高效且快速地处理小体积样本。虽然已经证明使用微流体系统能够从血液等复杂悬浮液中捕获稀有细胞，但很少有方法能实现对结合细胞的快速且无损释放。这种分离对于组织工程和基于细胞的治疗应用至关重要，这与诊断应用不同，在诊断中可以通过简单裂解捕获的细胞来进行免疫组织化学、蛋白质组学和基因组学分析。本文展示了在藻酸盐水凝胶中加入四臂胺封端的聚乙二醇（PEG）分子以及抗体如何能够增强水凝胶从全血中捕获内皮祖细胞（EPCs）的能力。水凝胶涂层共形地涂覆在微流体通道内的柱结构上，并且使用螯合剂溶解它们能够在捕获后有效地回收内皮祖细胞。
Microfluidic adhesion-based cell separation systems are of interest in clinical and biological applications where small sample volumes must be processed efficiently and rapidly. While the ability to capture rare cells from complex suspensions such as blood using microfluidic systems has been demonstrated, few methods exist for rapid and non-destructive release of the bound cells. Such detachment is critical for applications in tissue engineering and cell-based therapeutics in contrast with diagnostics wherein immunohistochemical, proteomic, and genomic analyses can be carried out by simply lysing captured cells. This paper demonstrates how the incorporation of 4-arm amine terminated poly(ethylene glycol) (PEG) molecules along with antibodies within alginate hydrogels can enhance the ability of the hydrogels to capture endothelial progenitor cells (EPCs) from whole human blood. The hydrogel coatings are applied conformally onto pillar structures within microfluidic channels and their dissolution with a chelator allows for effective recovery of EPCs following capture.
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影响因子：
56.9
作者：
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影响因子：
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影响因子：
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