Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H(2)O(2) Treatment.
Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H(2)O(2) Treatment.
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作者：
海藻多糖的高分子量和较差的溶解度限制了其功能和应用。以马尾藻为原料，采用紫外/过氧化氢（UV/H2O2）处理制备低分子量海藻多糖。研究了UV/H2O2处理对金丝桃多糖理化性质和抗光老化活性的影响。紫外/H2O2处理能有效降解梭菌多糖，使其分子量从271 kDa降至26 kDa。处理不影响ddsfp中的官能团，但改变了它们的单糖组成和形态的摩尔百分比。利用体外培养的人表皮HaCaT细胞，研究了不同处理对金针梅多糖抗光老化功能的影响。DFSPs显著提高了uvb辐照HaCaT细胞的细胞活力和羟脯氨酸分泌。其中UV/H2O2处理45 min得到的DSFP-45抗光老化效果最好。此外，DSFP-45显著提高了I型胶原的含量和表达，降低了促炎细胞因子，包括白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α的含量和表达。由此可见，UV/H2O2处理能有效提高金丝藤多糖的抗光老化活性。这些结果为利用海藻多糖开发新型高效抗光老化药物或功能性食品提供了一些启示。
The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H2O2) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme. The effects of UV/H2O2 treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H2O2 treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H2O2 treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H2O2 treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides.
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