Genome-Wide Identification of Rapid Alkalinization Factor Family in Brassica napus and Functional Analysis of BnRALF10 in Immunity to Sclerotinia sclerotiorum.
Genome-Wide Identification of Rapid Alkalinization Factor Family in Brassica napus and Functional Analysis of BnRALF10 in Immunity to Sclerotinia sclerotiorum.
复制标题
甘蓝型油菜快速碱化因子家族的全基因组鉴定及BnRALF10对核盘菌免疫的功能分析
DOI：
10.3389/fpls.2022.877404
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
快速碱化因子（RALF）是近年来发现的植物免疫调节因子。然而，信号转导的基础RALF触发的免疫作物物种对坏死营养型病原体仍然在很大程度上是未知的。本研究鉴定了油菜RALF家族成员，并揭示了BnRALF10在油菜抗核盘菌（Sclerotinia sclerotiorum）免疫中的功能及其信号转导机制。油菜基因组携带61个RALF，其中一半是非典型的，含有不太保守的YISY基序，缺乏RRXL基序或一对半胱氨酸。全家族的基因表达分析表明，表达模式响应于S。核盘菌感染和DAMP和PAMP处理通常是RALF和刺激特异性的。大多数显著应答的BnRALF基因被S.而BnPep5和SsNLP 1则下调了更多的BnRALF基因。这些结果表明，BnRALF家族成员可能差异参与植物免疫。功能分析表明，BnRALF10在油菜中引起多种免疫应答，并刺激对S.菌核这些数据支持BnRALF 10作为DAMP在植物免疫中发挥积极作用。BnRALF10与BnFER相互作用。BnFER基因沉默降低了BnRALF10诱导的活性氧（ROS）的产生，降低了油菜对S.菌核这些结果支持BnFER是BnRALF10的受体。此外，定量蛋白质组学分析鉴定了数十种BnRALF10引发的防御（RED）蛋白，这些蛋白质在蛋白质丰度上响应BnRALF10并在防御中发挥作用。我们的研究结果表明，BnRALF10调节RED蛋白的丰度，以微调植物免疫。总的来说，我们的研究结果提供了一些见解油菜RALF的功能和BnRALF触发免疫的信号基础。
Rapid alkalinization factors (RALFs) were recently reported to be important players in plant immunity. Nevertheless, the signaling underlying RALF-triggered immunity in crop species against necrotrophic pathogens remains largely unknown. In this study, RALF family in the important oil crop oilseed rape (Brassica napus) was identified and functions of BnRALF10 in immunity against the devastating necrotrophic pathogen Sclerotinia sclerotiorum as well as the signaling underlying this immunity were revealed. The oilseed rape genome carried 61 RALFs, half of them were atypical, containing a less conserved YISY motif and lacking a RRXL motif or a pair of cysteines. Family-wide gene expression analyses demonstrated that patterns of expression in response to S. sclerotiorum infection and DAMP and PAMP treatments were generally RALF- and stimulus-specific. Most significantly responsive BnRALF genes were expressionally up-regulated by S. sclerotiorum, while in contrast, more BnRALF genes were down-regulated by BnPep5 and SsNLP1. These results indicate that members of BnRALF family are likely differentially involved in plant immunity. Functional analyses revealed that BnRALF10 provoked diverse immune responses in oilseed rape and stimulated resistance to S. sclerotiorum. These data support BnRALF10 to function as a DAMP to play a positive role in plant immunity. BnRALF10 interacted with BnFER. Silencing of BnFER decreased BnRALF10-induced reactive oxygen species (ROS) production and compromised rape resistance to S. sclerotiorum. These results back BnFER to be a receptor of BnRALF10. Furthermore, quantitative proteomic analysis identified dozens of BnRALF10-elicited defense (RED) proteins, which respond to BnRALF10 in protein abundance and play a role in defense. Our results revealed that BnRALF10 modulated the abundance of RED proteins to fine tune plant immunity. Collectively, our results provided some insights into the functions of oilseed rape RALFs and the signaling underlying BnRALF-triggered immunity.
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DOI：
10.1126/science.1244454
发表时间：
2014-01-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Haruta M;Sabat G;Stecker K;Minkoff BB;Sussman MR
通讯作者：
Sussman MR
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
11.6
作者：
Hachez, Charles;Laloux, Timothee;Chaumont, Francois
通讯作者：
Chaumont, Francois
影响因子：
2.9
作者：
Haruta, Miyoshi;Monshausen, Gabriele;Sussman, Michael R.
通讯作者：
Sussman, Michael R.
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS