Complete genome analysis of the C4 subgenotype strains of enterovirus 71: predominant recombination C4 viruses persistently circulating in China for 14 years.
Complete genome analysis of the C4 subgenotype strains of enterovirus 71: predominant recombination C4 viruses persistently circulating in China for 14 years.
复制标题
肠道病毒71型C4亚基因型毒株的完整基因组分析：在中国持续传播14年的优势重组C4病毒
DOI：
10.1371/journal.pone.0056341
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Zhang Y;Tan X;Cui A;Mao N;Xu S;Zhu Z;Zhou J;Shi J;Zhao Y;Wang X;Huang X;Zhu S;Zhang Y;Tang W;Ling H;Xu W
基因重组是肠道病毒的一个众所周知的现象。为了解肠道病毒71型（EV 71）在中国的流行特征和重组可能性，对1998年以来中国手足口病（HFMD）大规模暴发和非暴发流行期间分离的16株EV 71进行了全基因组序列测定。将16株中国EV 71的全基因组序列与GenBank中1970-2011年的186株EV 71基因组序列进行比对，其中中国大陆104株，国外82株。对EV 71各亚型的最早株和HEV-A原型株进行系统发育分析和Simplot分析。系统发育分析表明，除HuB/CHN/2009和Xiamen/CHN/2009分别聚为A和B5亚型外，其余中国毒株均聚为C4亚型。大多数C4 EV 71可分为C4 b和C4 a两个进化分支。综合重组分析表明，C4亚型（包括C4 a和C4 B）结构基因与CAV 16、14和4原型株的非结构基因之间存在基因组重组，但C4毒株与B亚型之间存在基因组内重组的证据不够充分。该病毒于1998年首次在中国大陆发现，并成为中国大陆流行的主要地方性病毒，持续了至少14年。观察到C4重组病毒的C4 a和C4 b进化分支之间的转移，并且C4 a病毒与自2007年以来全国范围内的大规模手足口病爆发相关，具有较高的发病率和死亡率。
Genetic recombination is a well-known phenomenon for enteroviruses. To investigate the genetic characterization and the potential recombination of enterovirus 71 (EV71) circulating in China, we determined the 16 complete genome sequences of EV71 isolated from Hand Foot Mouth Disease (HFMD) patients during the large scale outbreak and non-outbreak years since 1998 in China. The full length genome sequences of 16 Chinese EV71 in present study were aligned with 186 genome sequences of EV71 available from GenBank, including 104 China mainland and 82 international sequences, covering the time period of 1970–2011. The oldest strains of each subgenotype of EV71 and prototype strains of HEV-A were included to do the phylogenetic and Simplot analysis. Phylogenetic analysis indicated that all Chinese strains were clustered into C4 subgenotype of EV71, except for HuB/CHN/2009 clustered into A and Xiamen/CHN/2009 clustered into B5 subgenotype. Most of C4 EV71 were clustered into 2 predominant evolutionary branches: C4b and C4a evolutionary brunches. Our comprehensive recombination analysis showed the evidence of genome recombination of subgenotype C4 (including C4a and C4b) sequences between structural genes from genotype C EV71 and non-structural genes from the prototype strains of CAV16, 14 and 4, but the evidence of intratypic recombination between C4 strains and B subgenotype was not enough strong. This intertypic recombination C4 viruses were first seen in 1998 and became the predominant endemic viruses circulating in China mainland for at least 14 years. A shift between C4a and C4b evolutionary brunches of C4 recombination viruses were observed, and C4a viruses have been associated with large scale nationwide HFMD outbreak with higher morbidity and mortality since 2007.
登录
查看更多内容
影响因子：
3.7
作者：
Tan X;Huang X;Zhu S;Chen H;Yu Q;Wang H;Huo X;Zhou J;Wu Y;Yan D;Zhang Y;Wang D;Cui A;An H;Xu W
通讯作者：
Xu W
影响因子：
5.4
作者：
MCGOLDRICK, A;MACADAM, AJ;ALMOND, JW
通讯作者：
ALMOND, JW
影响因子：
5.4
作者：
Brown, BA;Oberste, MS;Pallansch, MA
通讯作者：
Pallansch, MA
影响因子：
5.4
作者：
CHRISTODOULOU, C;COLBEREGARAPIN, F;HORAUD, F
通讯作者：
HORAUD, F
影响因子：
5
作者：
Huang, Yuan-Pin;Lin, Tsuey-Li;Wu, Ho-Sheng
通讯作者：
Wu, Ho-Sheng