Analysis of Sanitizer Rotation on the Susceptibility, Biofilm Forming Ability and Caco-2 Cell Adhesion and Invasion of Listeria.
Analysis of Sanitizer Rotation on the Susceptibility, Biofilm Forming Ability and Caco-2 Cell Adhesion and Invasion of Listeria.
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DOI：
10.3390/pathogens11090961
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发表时间：
2022-08-24
期刊：
影响因子：
--
通讯作者：
Yemmireddy V
中科院分区：
文献类型：
--
作者：
Rahman MA;Sahoo N;Yemmireddy V
本研究的目的是确定消毒剂使用条件对单核细胞增多性李斯特菌的敏感性、生物被膜形成能力和致病性的影响。用不同浓度(4~512ppm)的次氯酸钠、苯扎氯铵和过氧乙酸分别作用于两株不同的单核细胞增多性乳杆菌和一株非致病的诺氏乳杆菌，处理时间(30 S~5min)。在试验条件下，三种试验消毒剂的消毒量没有显著差异(p>；0.05)。根据消毒剂浓度和处理次数的不同，可以观察到减少1~8logCFU/mL。与亲本菌株相比，在特定消毒剂的最高亚致死浓度和处理时间下，再次接触相同或不同的消毒剂时，存活的细胞没有变化或敏感性增加。在反复暴露于浓度从1 ppm递增到128ppm的消毒剂后，微囊藻能够分别在浓度高达32ppm的苯扎氯铵和ppm过氧乙酸的处理下存活。在试验的亚致死浓度下，试验菌株之间在生物被膜形成方面没有显著差异(p>；0.05)。Caco-2与白纹伊蚊的相互作用表明，随着消毒剂的亚致死浓度，其侵染能力降低。
The purpose of this study was to determine the effect of sanitizer use conditions on the susceptibility, biofilm forming ability and pathogenicity of Listeria monocytogenes. Two different strains of L. monocytogenes and a non-pathogenic L. innocua were exposed to sodium hypochlorite, benzalkonium chloride and peroxyacetic acid at different concentrations (4 to 512 ppm) and treatment times (30 s to 5 min), respectively. Under the tested conditions, no significant difference (p > 0.05) in reduction was observed among the three tested sanitizers. A reduction of 1 to 8 log CFU/mL was observed depending upon the sanitizer concentration and treatment times. The survived cells at the highest sublethal concentration and treatment time of a particular sanitizer upon re-exposure to the same or different sanitizer showed either no change or increased susceptibility when compared to parent strains. Upon repeated exposure to sanitizers at progressively increasing concentrations from 1 to 128 ppm, L. innocua was able to survive concentrations of up to 32 ppm benzalkonium chloride and 64 ppm peroxyacetic acid treatments, respectively. At the tested sub-lethal concentrations, no significant difference (p > 0.05) in biofilm formation was observed among the tested strains. Caco-2 interaction with L. innocua showed a reduction in invasion ability with sublethal concentrations of sanitizers.
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影响因子：
5.2
作者：
Bland R;Waite-Cusic J;Weisberg AJ;Riutta ER;Chang JH;Kovacevic J
通讯作者：
Kovacevic J
影响因子：
4
作者：
Langsrud, S;Sundheim, G;Borgmann-Strahsen, R
通讯作者：
Borgmann-Strahsen, R
影响因子：
5.2
作者：
Hua, Zi;Korany, Ahmed Mahmoud;Zhu, Mei-Jun
通讯作者：
Zhu, Mei-Jun
影响因子：
6.4
作者：
Gullberg E;Albrecht LM;Karlsson C;Sandegren L;Andersson DI
通讯作者：
Andersson DI
影响因子：
--
作者：
Russo P;Botticella G;Capozzi V;Massa S;Spano G;Beneduce L
通讯作者：
Beneduce L