Adaptation to a Commercial Quaternary Ammonium Compound Sanitizer Leads to Cross-Resistance to Select Antibiotics in Listeria monocytogenes Isolated From Fresh Produce Environments.
Adaptation to a Commercial Quaternary Ammonium Compound Sanitizer Leads to Cross-Resistance to Select Antibiotics in Listeria monocytogenes Isolated From Fresh Produce Environments.
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DOI：
10.3389/fmicb.2021.782920
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发表时间：
2021
影响因子：
5.2
通讯作者：
Kovacevic J
中科院分区：
文献类型：
--
作者：
Bland R;Waite-Cusic J;Weisberg AJ;Riutta ER;Chang JH;Kovacevic J
通过清洁和卫生措施有效消除单核细胞增生李斯特菌对食品加工业至关重要。特别是在新鲜农产品操作中，缺乏杀灭步骤需要有效的清洁和卫生，以减轻环境交叉污染的风险。由于工厂依赖消毒剂来控制L。由于单核细胞增多症，通过交叉耐药性对消毒剂和其他抗菌剂产生耐受性的报告特别令人关切。我们研究了六个L的潜力。单核细胞增多症从新鲜产品处理和加工设施和包装车间分离，以在商业消毒剂和抗生素之间产生交叉抗性。将属于超毒力克隆复合物（CC 2、CC 4和CC 6）的分离株实验适应市售季铵化合物消毒剂（cQAC），导致最低抑菌浓度（2-3 ppm）和最低杀菌浓度（3-4 ppm）升高。在利血平（一种已知的外排泵抑制剂）存在下，所有适应性（qAD）分离株对cQAC的敏感性恢复。在所有测试的分离株中观察到对7/17种测试的抗生素（氯霉素、环丙沙星、克林霉素、卡那霉素、新生霉素、青霉素和链霉素）的敏感性降低。qAD分离株仍然对通常用于治疗淋病的抗生素敏感（即，氨苄青霉素和庆大霉素）。qAD菌株的全基因组测序，然后进行比较基因组分析，揭示了FepA氟喹诺酮外排泵的调节因子fepR中的几个突变。结果表明，fepR突变在对cQAC低水平适应后抗生素敏感性降低中发挥作用。进一步探讨了该现象的发生机制和产生的压力。需要从不同来源回收的单核细胞增生分离株，以更好地了解食物链分离株中交叉耐药性发展的可能性以及对食品工业的影响。
The effective elimination of Listeria monocytogenes through cleaning and sanitation is of great importance to the food processing industry. Specifically in fresh produce operations, the lack of a kill step requires effective cleaning and sanitation to mitigate the risk of cross-contamination from the environment. As facilities rely on sanitizers to control L. monocytogenes, reports of the development of tolerance to sanitizers and other antimicrobials through cross-resistance is of particular concern. We investigated the potential for six L. monocytogenes isolates from fresh produce handling and processing facilities and packinghouses to develop cross-resistance between a commercial sanitizer and antibiotics. Experimental adaptation of isolates belonging to hypervirulent clonal complexes (CC2, CC4, and CC6) to a commercial quaternary ammonium compound sanitizer (cQAC) resulted in elevated minimum inhibitory concentrations (2–3 ppm) and minimum bactericidal concentrations (3–4 ppm). Susceptibility to cQAC was restored for all adapted (qAD) isolates in the presence of reserpine, a known efflux pump inhibitor. Reduced sensitivity to 7/17 tested antibiotics (chloramphenicol, ciprofloxacin, clindamycin, kanamycin, novobiocin, penicillin, and streptomycin) was observed in all tested isolates. qAD isolates remained susceptible to antibiotics commonly used in the treatment of listeriosis (i.e., ampicillin and gentamicin). The whole genome sequencing of qAD strains, followed by comparative genomic analysis, revealed several mutations in fepR, the regulator for FepA fluoroquinolone efflux pump. The results suggest that mutations in fepR play a role in the reduction in antibiotic susceptibility following low level adaptation to cQAC. Further investigation into the cross-resistance mechanisms and pressures leading to the development of this phenomenon among L. monocytogenes isolates recovered from different sources is needed to better understand the likelihood of cross-resistance development in food chain isolates and the implications for the food industry.
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影响因子：
56.3
作者：
de Noordhout, Charline Maertens;Devleesschauwer, Brecht;Angulo, Frederick J.;Verbeke, Geert;Haagsma, Juanita;Kirk, Martyn;Havelaar, Arie;Speybroeck, Niko
通讯作者：
Speybroeck, Niko
影响因子：
4.4
作者：
Elhanafi, Driss;Dutta, Vikrant;Kathariou, Sophia
通讯作者：
Kathariou, Sophia
影响因子：
4.9
作者：
Godreuil, S;Galimand, M;Courvalin, P
通讯作者：
Courvalin, P
影响因子：
4.2
作者：
Cherifi, Tamazight;Carrillo, Catherine;Fravalo, Philippe
通讯作者：
Fravalo, Philippe
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.