Interstitial Cajal-like cells (ICLC) as steroid hormone sensors in human myometrium: immunocytochemical approach.
Interstitial Cajal-like cells (ICLC) as steroid hormone sensors in human myometrium: immunocytochemical approach.
复制标题
DOI：
10.1111/j.1582-4934.2006.tb00438.x
复制
发表时间：
2006-07
影响因子：
5.3
通讯作者：
Ardeleanu C
中科院分区：
文献类型：
--
作者：
Cretoiu D;Ciontea SM;Popescu LM;Ceafalan L;Ardeleanu C
研究了培养的人正常子宫肌层细胞（非妊娠子宫、生育期）中雌激素（ER）和孕激素（PR）受体的表达。采用免疫组织化学和免疫荧光法检测心肌细胞和间质Cajal样细胞（ICLC）ER和PR的表达。只有那些c-kit和类固醇受体双重免疫染色的细胞被认为是ICLC。ER和/或PR免疫反应性定位于ICLC，主要集中在细胞核水平，但也观察到在细胞体（细胞质）和过程。ICLC细胞核PR免疫阳性反应强于ER。在我们的实验条件下，在肌细胞中无法检测到类固醇受体的明显阳性可重复反应。总之，这些数据表明，ICLC可能是真正的激素“传感器”，可能参与调节人类子宫肌层收缩（通过与肌细胞的间隙连接和/或旁分泌信号）。
Expression of estrogen (ER) and progesterone (PR) receptors was investigated in cultured human normal myometrial cells (non-pregnant uterus, fertile period). The ER and PR expression was studied by immunohistochemistry and immunofluorescence on either myocytes or interstitial Cajal-like cells (ICLC). Only those cells double immunostained for c-kit and steroid receptors were considered as ICLC. ER and/or PR immunoreactivity was localized in ICLC, primarily concentrated at the nucleus level, but it was also observed in the cell body (cytoplasm) and processes. Stronger immunopositive reaction in the ICLC nucleus for PR than for ER was noted. Under our experimental conditions, a clear positive repeatable reaction for steroid receptors could not be detected in myocytes. In conclusion, these data suggest that ICLC could be true hormonal ‘sensors’, possibly participating in the regulation of human myometrial contractions (via gap junctions with myocytes and/or by paracrine signaling).
登录
查看更多内容
影响因子：
5.5
作者：
Beckett, Elizabeth A. H.;McCloskey, Conor;Koh, Sang Don
通讯作者：
Koh, Sang Don
DOI：
10.1111/j.1582-4934.2006.tb00410.x
发表时间：
2006-04
影响因子：
5.3
作者：
Popescu LM;Gherghiceanu M;Hinescu ME;Cretoiu D;Ceafalan L;Regalia T;Popescu AC;Ardeleanu C;Mandache E
通讯作者：
Mandache E
影响因子：
5.5
作者：
Hashitani, Hikaru
通讯作者：
Hashitani, Hikaru
DOI：
10.1152/ajpgi.2001.280.2.g255
发表时间：
2001-02-01
影响因子：
4.5
作者：
Aytug, N;Giral, A;Ulusoy, NB
通讯作者：
Ulusoy, NB
影响因子：
5.5
作者：
Sanders, Kenton M.;Ward, Sean M.
通讯作者：
Ward, Sean M.