Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
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DOI：
10.1111/j.1582-4934.2006.tb00410.x
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发表时间：
2006-04
影响因子：
5.3
通讯作者：
Mandache E
中科院分区：
文献类型：
--
作者：
Popescu LM;Gherghiceanu M;Hinescu ME;Cretoiu D;Ceafalan L;Regalia T;Popescu AC;Ardeleanu C;Mandache E
我们以前描述了人类心房肌间质Cajal样细胞（ICLC）。使用了几种补充方法来验证大鼠或人心室肌细胞中ICLC的存在：原代细胞培养、活体染色（例如：亚甲蓝）、传统染色（包括银浸渍）、相差和非常规光学显微镜（Epon包埋的半薄切片）、透射电子显微镜（TEM）（连续切片）、体视学、免疫组织化学（IHC）和使用分子探针的免疫荧光（IF）。心肌细胞约占大鼠心室肌体积的75%。ICLC占间质细胞数量的约32%，心肌细胞/ICLC的比例约为70/1。在突触中，ICLC与神经纤维、肌细胞、毛细血管和免疫反应细胞（间质突触）建立密切联系。ICLC显示特征性的胞质突起，通常为两个或三个，非常长（数十至数百μm），非常薄（0.1-0.5μm厚），口径不均匀，具有扩张，导致念珠状外观。这些突起之间存在缝隙连接，通常由线粒体（如Janus绿色B和Mito Tracker绿色FM所示）和内质网元件占据。典型的是，一些植被是平坦的，具有面纱状的外观，形成了一个亚热带系统。ICLC细胞膜上有小窝（约1个小窝/1μm细胞膜，占细胞质体积的2-4%），线粒体和内质网（粗面和光面）分别占细胞质体积的5-10%和1-2%。免疫组化显示CD 34、EGFR和波形蛋白阳性，仅在少数病例中显示CD 117阳性。IHC对结蛋白、CD 57、tau、糜蛋白酶、类胰蛋白酶和CD 13呈阴性。IF显示心室ICLC表达连接蛋白43。我们可以推测，可能的ICLC的作用可能是：细胞间信号（神经元，肌细胞，毛细血管等）。和/或化学机械传感器。在病理学方面，ICLC可能参与心脏修复/重塑、心肌发生和最终猝死的过程似乎很有吸引力。
We have previously described interstitial Cajal-like cells (ICLC) in human atrial myocardium. Several complementary approaches were used to verify the existence of ICLC in the interstitium of rat or human ventricular myocardium: primary cell cultures, vital stainings (e.g.: methylene blue), traditional stainings (including silver impregnation), phase contrast and non-conventional light microscopy (Epon-embedded semithin sections), transmission electron microscopy (TEM) (serial ultrathin sections), stereology, immunohistochemistry (IHC) and immunofluorescence (IF) with molecular probes. Cardiomyocytes occupy about 75% of rat ventricular myocardium volume. ICLC represent ∼32% of the number of interstitial cells and the ratio cardiomyocytes/ICLC is about 70/1. In the interstitium, ICLC establish close contacts with nerve fibers, myocytes, blood capillaries and with immunoreactive cells (stromal synapses). ICLC show characteristic cytoplasmic processes, frequently two or three, which are very long (tens up to hundreds of μm), very thin (0.1-0.5μm thick), with uneven caliber, having dilations, resulting in a moniliform aspect. Gap junctions between such processes can be found. Usually, the dilations are occupied by mitochondria (as revealed by Janus green B and Mito Tracker Green FM) and elements of endoplasmic reticulum. Characteristically, some prolongations are flat, with a veil-like appearance, forming a labyrinthic system. ICLC display caveolae (about 1 caveola/1μm cell membrane length, or 2-4% of the relative cytoplasmic volume, Mitochondria and endoplasmic reticulum (rough and smooth) occupy 5-10% and 1-2% of cytoplasmic volume, respectively. IHC revealed positive staining for CD34, EGFR and vimentin and, only in a few cases for CD117. IHC was negative for: desmin, CD57, tau, chymase, tryptase and CD13. IF showed that ventricular ICLC expressed connexin 43. We may speculate that possible ICLC roles might be: intercellular signaling (neurons, myocytes, capillaries etc.) and/or chemomechanical sensors. For pathology, it seems attractive to think that ICLC might participate in the process of cardiac repair/remodeling, arrhythmogenesis and, eventually, sudden death.
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DOI：
10.1111/j.1582-4934.2006.tb00306.x
发表时间：
2006-01
影响因子：
5.3
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
通讯作者：
Popescu LM
影响因子：
3.6
作者：
Duquette, RA;Shmygol, A;Wray, S
通讯作者：
Wray, S
影响因子：
3.6
作者：
Bobryshev, YV
通讯作者：
Bobryshev, YV
影响因子：
2.3
作者：
Cho, Woo Jung;Daniel, Edwin E.
通讯作者：
Daniel, Edwin E.
DOI：
10.1016/s0022-5320(74)90074-4
发表时间：
1974-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
ARLUK, DJ;RHODIN, JAG
通讯作者：
RHODIN, JAG