Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip.
Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip.
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DOI：
10.1021/jacs.6b12236
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发表时间：
2017-02-22
影响因子：
15
通讯作者：
Hammond PT
中科院分区：
文献类型：
--
作者：
Park MH;Reátegui E;Li W;Tessier SN;Wong KH;Jensen AE;Thapar V;Ting D;Toner M;Stott SL;Hammond PT
检测癌症患者血液中的罕见循环肿瘤细胞（CTC）有可能成为检查转移、评估预后、评估肿瘤对药物的敏感性和监测治疗结果的强大且非侵入性的方法。在这项研究中，我们开发了一种有效的策略，使用微流体免疫亲和方法从乳腺癌患者的血液中分离CTC。此外，为了进一步获得这些稀有细胞用于下游表征，我们的策略允许捕获的CTC从基底上容易地分离，而不损害细胞活力或采用下一代RNA测序鉴定特定乳腺癌基因的能力。为了实现这一点，化学配体交换反应被设计为释放附着在与人字形微流体芯片（NP-HBCTC-Chip）表面结合的金纳米颗粒涂层上的细胞。与使用未修饰的HBCTC芯片相比，我们的方法提供了几个优点，包括增强的捕获效率和分离的CTC的回收。
The detection of rare circulating tumor cells (CTCs) in the blood of cancer patients has the potential to be a powerful and noninvasive method for examining metastasis, evaluating prognosis, assessing tumor sensitivity to drugs, and monitoring therapeutic outcomes. In this study, we have developed an efficient strategy to isolate CTCs from the blood of breast cancer patients using a microfluidic immune-affinity approach. Additionally, to gain further access to these rare cells for downstream characterization, our strategy allows for easy detachment of the captured CTCs from the substrate without compromising cell viability or the ability to employ next generation RNA sequencing for the identification of specific breast cancer genes. To achieve this, a chemical ligand-exchange reaction was engineered to release cells attached to a gold nanoparticle coating bound to the surface of a herringbone microfluidic chip (NP-HBCTC-Chip). Compared to the use of the unmodified HBCTC-Chip, our approach provides several advantages, including enhanced capture efficiency and recovery of isolated CTCs.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
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作者：
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