Analysis of mechanisms of T-2 toxin toxicity using yeast DNA microarrays.
Analysis of mechanisms of T-2 toxin toxicity using yeast DNA microarrays.
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DOI：
10.3390/ijms9122585
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发表时间：
2008-12
影响因子：
5.6
通讯作者：
Iwahashi, Hitoshi
中科院分区：
文献类型：
--
作者：
Iwahashi, Yumiko;Kitagawa, Emiko;Iwahashi, Hitoshi
T-2毒素是一种真菌毒素，属于农产品中发现的A型三茂铁烯类化合物。通过对T-2毒素攻击后酵母转录组的分析来表征T-2毒素的细胞毒性。有趣的是，T-2毒素诱导的酵母基因表达谱被发现与放线菌亚胺处理后的谱相似。此外，T-2毒素处理被发现激活促进器、糖异生和细胞停滞相关基因，如丝裂原活化蛋白激酶基因(FUS3)。T-2毒素侵袭细胞膜，导致膜转运系统受到干扰。大量的基因被诱导恢复T-2毒素的毒性。然而，这些数据并不表明DNA损伤通过烷基化(Mag1，一种基因3-甲基腺嘌呤DNA糖基酶，0.46倍下调)，没有诱导DNA修复机制如重组(RAD26，RAD52等)。切除修复(RAD7、RAD14、RAD16、RAD23等)。这些结果表明，T-2毒素的毒性是由于其对酵母细胞细胞膜的干扰所致，T-2毒素具有轻微的致突变作用。
T-2 toxin is a mycotoxin that belongs to a group of type A tricothecenes found in agricultural products. The cytotoxicity of T-2 toxin was characterized by analysis of the yeast transcriptome upon challenge with T-2 toxin. Interestingly, T-2 toxin-induced yeast gene expression profiles were found to be similar to profiles obtained following cycloheximide treatment. Moreover, T-2 toxin treatment was found to activate facilitators, gluconeogenesis and cell arrest related genes such as mitogen-activated protein kinase genes (FUS3). T-2 toxin attacks the membrane and as a result the membrane transport system was disturbed. A large number of genes are induced to restore the toxicity caused by T-2 toxin. However, the data did not suggest that DNA damage by alkylation (Mag1, a gene 3-methyl-adenine DNA glycosylase, 0.46-fold down regulated), no induction of DNA repair mechanisms such as recombination (RAD26, RAD52 and etc.) and excision repair (RAD7, RAD14, RAD16, RAD23 and etc.). These results suggested that the toxicity of the T-2 toxin was due to the disturbance of the cell membrane of the yeast cell and that T-2 toxin caused mild mutagenesis.
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影响因子：
4.4
作者：
Iwahashi H;Kitagawa E;Suzuki Y;Ueda Y;Ishizawa YH;Nobumasa H;Kuboki Y;Hosoda H;Iwahashi Y
通讯作者：
Iwahashi Y
影响因子：
56.9
作者：
Butty, AC;Pryciak, PM;Peter, M
通讯作者：
Peter, M
影响因子：
56.9
作者：
PETER, M;HERSKOWITZ, I
通讯作者：
HERSKOWITZ, I
影响因子：
3.3
作者：
Santt, Olivier;Pfirrmann, Thorsten;Wolf, Dieter H.
通讯作者：
Wolf, Dieter H.
影响因子：
--
作者：
Sehata, S;Kiyosawa, N;Doi, K
通讯作者：
Doi, K