Changes in cardiovascular parameters in rats exposed to chronic widespread mechanical allodynia induced by hind limb cast immobilization.
Changes in cardiovascular parameters in rats exposed to chronic widespread mechanical allodynia induced by hind limb cast immobilization.
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DOI：
10.1371/journal.pone.0245544
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Sato J
中科院分区：
文献类型：
--
作者：
Yoshimoto T;Sakurai H;Ohmichi Y;Ohmichi M;Morimoto A;Ushida T;Sato J
为了阐明慢性疼痛条件与石膏固定和自主神经功能之间的关系，我们研究了清醒大鼠的自主神经系统的功能变化与慢性石膏固定后疼痛（CPCP）由两周的一条后肢石膏固定。我们遥测检查收缩期动脉血压（SBP），心率（HR）的时间过程中，从功率谱分析SBP变异性作为血管交感神经指数的中频（MF）组件。我们还研究了压力感受性反射对酚妥拉明（α-肾上腺素受体拮抗剂）的敏感性，以及对低环境温度（LT; 9.0 ± 0.2°C）暴露（交感神经兴奋剂）的SBP和HR反应。暴露于石膏固定的大鼠在双侧后肢小腿区域（皮肤和肌肉）的石膏去除后表现出持续至少10周的机械性异常性疼痛。在静息状态下，管型固定期间SBP、HR和MF组分显著增加（所有p < 0.001）。在移除石膏后，这些参数逐渐降低，并在1周内达到低于基线水平，持续超过10周。酚妥拉明（10 mg/kg，腹腔注射）显著降低了石膏固定前和固定期间的SBP（前，p < 0.001;期间，p = 0.001），但没有降低石膏取出后的SBP。酚妥拉明给药后的压力反射增益，计算为HR增加除以SBP降低，在管型去除后显著增加（p = 0.002）。LT暴露的SBP增加显着大于管型拆除后比管型固定前，表明交感神经递质的超敏反应。这些结果表明，在CPCP模型中，交感神经激活在石膏固定期间增强，然后在石膏去除后降低，并保持低于正常水平，持续疼痛行为。此外，在CPCP模型中，自主神经系统的反应性受损。
To elucidate the relationship between chronic pain conditions with cast immobilization and autonomic function, we investigated the functional changes of the autonomic nervous system in conscious rats with chronic post-cast pain (CPCP) induced by a two-week cast immobilization of one hind limb. We telemetrically examined the time courses of systolic arterial blood pressure (SBP), heart rate (HR), and the middle-frequency (MF) component obtained from the power spectral analysis of SBP variability as a vasomotor sympathetic index. We also investigated the baroreflex sensitivity to phentolamine, an α-adrenoceptor antagonist, and the SBP and HR responses to a low ambient temperature (LT; 9.0 ± 0.2°C) exposure, a sympathetic stimulant. Rats exposed to cast immobilization exhibited mechanical allodynia lasting for at least 10 weeks after cast removal in the calf area (skin and muscle) of the bilateral hind limbs. Under resting conditions, the SBP, HR, and MF components were significantly increased during cast immobilization (all p < 0.001). Following cast removal, these parameters gradually decreased and within 1 week reached lower than baseline levels, lasting for over 10 weeks. Phentolamine administration (10 mg/kg, intraperitoneally) significantly decreased the SBP before and during cast immobilization (before, p < 0.001; during, p = 0.001) but did not lower the SBP after cast removal. The baroreflex gain after phentolamine administration, calculated as the HR increase divided by the SBP reduction, was significantly increased after cast removal (p = 0.002). The SBP increase on LT exposure was significantly greater after cast removal than that before cast immobilization, suggesting hypersensitivity to sympathetic neurotransmitters. These results revealed that, in the CPCP model, sympathetic activation was augmented during cast immobilization, which then decreased after cast removal and remained below normal levels with persisting pain behaviors. Additionally, the responsiveness of the autonomic nervous system was impaired in the CPCP model.
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影响因子：
2.7
作者：
Schürmann, M;Gradl, G;Andress, HJ
通讯作者：
Andress, HJ
影响因子：
7.4
作者：
Gemes, Geza;Rigaud, Marcel;Seagard, Jeanne
通讯作者：
Seagard, Jeanne
DOI：
10.1016/j.autneu.2011.12.004
发表时间：
2012-04-03
期刊：
Autonomic neuroscience : basic & clinical
影响因子：
--
作者：
Oliveira LR;de Melo VU;Macedo FN;Barreto AS;Badaue-Passos D Jr;Viana dos Santos MR;Dias DP;Sluka KA;DeSantana JM;Santana-Filho VJ
通讯作者：
Santana-Filho VJ
影响因子：
7.4
作者：
Drummond, Peter D.;Drummond, Eleanor S.;Phillips, Jacqueline K.
通讯作者：
Phillips, Jacqueline K.
影响因子：
56.9
作者：
SATO, J;PERL, ER
通讯作者：
PERL, ER