Effect of indomethacin on alteration of ATPase-positive Langerhans cell density and cutaneous sunburn reaction induced by ultraviolet-B radiation.
Effect of indomethacin on alteration of ATPase-positive Langerhans cell density and cutaneous sunburn reaction induced by ultraviolet-B radiation.
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吲哚美辛对 ATP 酶阳性朗格汉斯细胞密度改变和 UV-B 辐射诱导的皮肤晒伤反应的影响。
DOI：
10.1111/1523-1747.ep12522659
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Gigli,I
中科院分区：
文献类型：
--
作者：
Lim,HW;Thorbecke,GJ;Baer,RL;Gigli,I
我们研究了紫外线b （UVB）照射对表皮atp酶阳性朗格汉斯细胞密度的影响，以及吲哚美辛（IND）对这种影响的调节。将脱毛的白化豚鼠背部暴露于不同剂量的UVB （10-550 mJ/cm2）。连续进行皮肤活检。树突状表皮朗格汉斯细胞的密度呈UVB剂量依赖性下降，这是通过其膜atp酶活性来确定的。这伴随着树枝状突起变薄、缩短或消失。这些变化之后，细胞密度在第21天逐渐恢复到辐照前水平。尽管给予了高剂量的UVB，但atp酶阳性细胞的密度最大下降仅为58%。照射后局部应用前列腺素合成酶抑制剂IND可减轻红斑；但仍观察到atp酶阳性细胞密度下降。相比之下，在照射前局部接受IND的豚鼠显示红斑反应降低，但没有显示atp酶阳性细胞密度的任何降低。在UVB暴露前3天口服IND并不能阻止细胞密度的下降。在照射前外用IND的保护作用，可以用它在中波紫外线和长波紫外线范围内的体外吸收来解释。2名受试者在暴露于UVB前20分钟局部应用IND，导致最小红斑剂量增加，防晒系数为1.6，与等量摩尔浓度的对氨基苯甲酸溶液产生的防晒系数相当。IND的防晒性能及其作为前列腺素合成酶抑制剂的活性表明，它可能是一种有用的防晒剂。然而，其临床安全性和有效性仍有待确定。
We have investigated the effect of ultraviolet-B (UVB) irradiation on the density of epidermal ATPase-positive Langerhans cells, and the modulation of this effect by indomethacin (IND). Depilated backs of albino guinea pigs were exposed to varying doses of UVB (10–550 mJ/cm2). Skin biopsies were taken serially. There was an UVB dose-dependent decrease in the density of dendritic epidermal Langerhans cells, as identified by their membrane ATPase activity. This was accompanied by thinning and shortening, or disappearance of dendritic processes. Such changes were followed by a gradual recovery of the cell density to preirradiation level by day 21. Despite the high doses of UVB given, the maximal decrease in the density of ATPase-positive cells was only 58%. Topical application of IND, a prostaglandin-synthetase inhibitor, after irradiation resulted in a decrease of the erythema; however, the decrease in the density of ATPase-positive cells was still observed. In contrast, guinea pigs that received IND topically prior to irradiation showed a decreased erythemal response, but failed to show any decrease in the density of ATPase-positive cells. Administration of IND orally for 3 days prior to UVB exposure did not prevent the decrease in the cell density.The protective effect of topical IND, applied prior to irradiation, may be explained by its in vitro absorbance at both the UVB and UVA ranges. Topical application of IND 20 min prior to exposure to UVB in 2 human subjects resulted in an increase in the minimal erythema dose, giving a sun protection factor of 1.6, which is comparable to that produced by an equimolar concentration of para-aminobenzoic acid solution. The sun-protective property of IND, together with its activity as a prostaglandin synthetase inhibitor, indicate that it potentially could be a useful sunscreen agent. Its clinical safety and efficacy, however, remain to be determined.
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DOI：
10.1038/jid.1949.6
发表时间：
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期刊：
The Journal of investigative dermatology
影响因子：
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DOI：
10.1111/1523-1747.ep12493242
发表时间：
1981
期刊：
The Journal of investigative dermatology
影响因子：
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作者：
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