Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity
Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity
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分析进化保守模块对 HMW-GS 1Ax1 启动子活性的作用
DOI：
10.1007/s11103-019-00943-6
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发表时间：
2019-12
影响因子：
5.1
通讯作者：
Xiaohui Li
中科院分区：
文献类型：
--
作者：
Luning Duan;Shichen Han;Ke Wang;Peihong Jiang;Yunsong Gu;Lin Chen;Junyi Mu;Xingguo Ye;Yaxuan Li;Yueming Yan;Xiaohui Li
不同的启动子区决定了1Ax 1的特异性和高水平表达。HMW-GS的合成发生在糊粉层细胞中。杂合蛋白可以储存在蛋白体中。高分子量麦谷蛋白亚基（HMW-GS）在小麦及近缘物种的胚乳中高度表达，其表达水平和等位基因变异影响面包制作品质和面粉的营养品质。然而，调节HMW-GS表达的机制仍不清楚。在这项研究中，我们分析了顺式作用元件的分布在2659 bp的启动子区的HMW-GS基因1Ax 1，它可以分为5个元素富集区。利用5'端进行性缺失片段驱动GUS基因在转基因小麦中表达，并通过组织化学染色证实了GUS基因在糊粉层细胞、内淀粉胚乳细胞、淀粉胚乳转移细胞和糊粉转移细胞中的表达。从-297到-1的启动子区域负责组织特异性表达，而从-1724到-618和从-618到-297的片段负责高水平表达。在1Ax 1启动子的调控下，即使没有特定的定位信号，外源蛋白也能以蛋白体的形式储存在胚乳细胞内。我们的研究结果不仅加深了我们对麦谷蛋白表达调控、运输和积累的理解，而且为利用小麦胚乳作为生物反应器生产营养物质和代谢产物提供了策略。
The specific and high-level expression of 1Ax1 is determined by different promoter regions. HMW-GS synthesis occurs in aleurone layer cells. Heterologous proteins can be stored in protein bodies. High-molecular-weight glutenin subunit (HMW-GS) is highly expressed in the endosperm of wheat and relative species, where their expression level and allelic variation affect the bread-making quality and nutrient quality of flour. However, the mechanism regulating HMW-GS expression remains elusive. In this study, we analyzed the distribution of cis-acting elements in the 2659-bp promoter region of the HMW-GS gene 1Ax1, which can be divided into five element-enriched regions. Fragments derived from progressive 5' deletions were used to drive GUS gene expression in transgenic wheat, which was confirmed in aleurone layer cells, inner starchy endosperm cells, starchy endosperm transfer cells, and aleurone transfer cells by histochemical staining. The promoter region ranging from - 297 to - 1 was responsible for tissue-specific expression, while fragments from - 1724 to - 618 and from - 618 to - 297 were responsible for high-level expression. Under the control of the 1Ax1 promoter, heterologous protein could be stored in the form of protein bodies in inner starchy endosperm cells, even without a special location signal. Our findings not only deepen our understanding of glutenin expression regulation, trafficking, and accumulation but also provide a strategy for the utilization of wheat endosperm as a bioreactor for the production of nutrients and metabolic products.
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影响因子：
11.4
作者：
Rushton, PJ;Torres, JT;Somssich, IE
通讯作者：
Somssich, IE
DOI：
10.4161/gmcr.27371
发表时间：
2014-01
期刊：
GM crops & food
影响因子：
--
作者：
Thilmony R;Guttman ME;Lin JW;Blechl AE
通讯作者：
Blechl AE
影响因子：
2.4
作者：
S. Uthayakumaran;S. Uthayakumaran;H. Beasley;F. Stoddard;M. Keentok;M. Keentok;Nhan Phan-Thien
通讯作者：
S. Uthayakumaran;S. Uthayakumaran;H. Beasley;F. Stoddard;M. Keentok;M. Keentok;Nhan Phan-Thien
影响因子：
13.8
作者：
Jian-xiang Wu;Lian Yu;Long Li;Jinqiang Hu;Jiyong Zhou;Xue-ping Zhou
通讯作者：
Jian-xiang Wu;Lian Yu;Long Li;Jinqiang Hu;Jiyong Zhou;Xue-ping Zhou
影响因子：
7.3
作者：
H. L. Wang;J. Patrick;C. Offler;X. Wang
通讯作者：
H. L. Wang;J. Patrick;C. Offler;X. Wang