Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses.
Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses.
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噬菌体展示技术在冠状病毒诊断和抗体选择中的应用。
DOI：
10.1007/s00284-021-02398-9
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发表时间：
2021-04
影响因子：
2.6
通讯作者：
Tripathi BN
中科院分区：
文献类型：
--
作者：
Anand T;Virmani N;Bera BC;Vaid RK;Vashisth M;Bardajatya P;Kumar A;Tripathi BN
噬菌体展示技术自1985年被发现以来，一直是分子生物学研究领域不可缺少的重要技术之一。由于大量的核苷酸片段可以克隆到噬菌体基因组中，噬菌体文库可能含有数百万或有时数十亿独特和独特的展示肽配体。配体-受体相互作用形成噬菌体展示的基础，已经被很好地利用在噬菌体表面的表位作图和抗原呈递，用于通过使用称为生物淘选的基于亲和选择的策略来筛选新的疫苗候选物。在过去的三十年里，这种多功能的技术得到了极大的改进，导致了用于组合肽展示的不同平台的产生。由此开发的新诊断工具的翻译已用于由病原微生物引起的情况，包括细菌和致命病毒，如寨卡病毒、埃博拉病毒、亨德拉病毒、尼帕病毒、汉坦病毒、中东呼吸综合征和SARS。在当前冠状病毒疾病（COVID-19）大流行的情况下，寻找中和抗体是研究人员寻找针对新型SARS-CoV-2的治疗候选物的动力。噬菌体展示技术是筛选抗体的重要技术，本文就噬菌体展示技术在冠状病毒疾病诊断和治疗中的应用作一简要综述。希望这项技术可以补充宿主-病原体相互作用的研究，并协助冠状病毒药物发现的新策略。
Phage display is one of the important and effective molecular biology techniques and has remained indispensable for research community since its discovery in the year 1985. As a large number of nucleotide fragments may be cloned into the phage genome, a phage library may harbour millions or sometimes billions of unique and distinctive displayed peptide ligands. The ligand–receptor interactions forming the basis of phage display have been well utilized in epitope mapping and antigen presentation on the surface of bacteriophages for screening novel vaccine candidates by using affinity selection-based strategy called biopanning. This versatile technique has been modified tremendously over last three decades, leading to generation of different platforms for combinatorial peptide display. The translation of new diagnostic tools thus developed has been used in situations arising due to pathogenic microbes, including bacteria and deadly viruses, such as Zika, Ebola, Hendra, Nipah, Hanta, MERS and SARS. In the current situation of pandemic of Coronavirus disease (COVID-19), a search for neutralizing antibodies is motivating the researchers to find therapeutic candidates against novel SARS-CoV-2. As phage display is an important technique for antibody selection, this review presents a concise summary of the very recent applications of phage display technique with a special reference to progress in diagnostics and therapeutics for coronavirus diseases. Hopefully, this technique can complement studies on host–pathogen interactions and assist novel strategies of drug discovery for coronaviruses.
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