Genetic analysis and gene fine mapping of a rolling leaf mutant (rl11(t)) in rice (Oryza sativa L.)

Genetic analysis and gene fine mapping of a rolling leaf mutant (rl11(t)) in rice (Oryza sativa L.)
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水稻卷叶突变体(rl11(t))的遗传分析和基因精细定位(Oryza sativa L.)

DOI:
10.1007/s11434-010-3137-0
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发表时间:
2010-06
期刊:
科学通报(英文版)
影响因子:
--
通讯作者:
梁国华
梁国华
中科院分区:
其他
文献类型:
--
作者:
梁国华

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寻找控制水稻叶形的新基因是水稻功能基因组学和株型改良的重要基础。在本研究中,我们从水稻品种粤丰B中筛选到一个卷叶突变体rl11(T),该突变体表现出株高降低,叶片卷曲,叶片变窄。与野生型植株相比,rl11(T)突变体的叶片具有异常的叶脉数量和形态。此外,突变体rl11(T)对生长素的抑制作用不如野生型敏感。遗传分析表明,该突变体受1对隐性基因控制。初步将rl11(T)基因定位于第4染色体上的SSR标记RM6089和RM124之间,并开发了32个新的STS标记用于精细定位。构建了包含rl11(T)基因座的物理图谱,最终将目的基因定位在BAC AL606645上STS4-25和STS4-26之间的31.6kb范围内。这为克隆rl11(T)基因提供了有用的信息。
The exploration of new genes controlling rice leaf shape is an important foundation for rice functional genomics and plant architecture improvement. In the present study, we identified a rolling leaf mutant from indica variety Yuefeng B, named rl11(t), which exhibited reduced plant height, rolling and narrow leaves. Leaves in rl11(t) mutant showed abnormal number and morphology of veins compared with those in wild type plants. In addition, rl11(t) mutant was less sensitive to the inhibitory effect of auxin than the wild type. Genetic analysis suggested that the mutant was controlled by a single recessive gene. Gene rl11(t) was initially mapped between SSR markers RM6089 and RM124 on chromosome 4. Thirty-two new STS markers around the rl11(t) region were developed for fine mapping. A physical map encompassing the rl11(t) locus was constructed and the target gene was finally delimited to a 31.6 kb window between STS4-25 and STS4-26 on BAC AL606645. This provides useful information for cloning of rl11(t) gene.
水稻窄叶突变体的遗传分析和基因定位(Oryza sativa L.)
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发表时间: 2009-02
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