Pronounced inflammatory response to endotoxaemia during nighttime: a randomised cross-over trial.
Pronounced inflammatory response to endotoxaemia during nighttime: a randomised cross-over trial.
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DOI：
10.1371/journal.pone.0087413
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gögenur I
中科院分区：
文献类型：
--
作者：
Alamili M;Bendtzen K;Lykkesfeldt J;Rosenberg J;Gögenur I
身体功能的昼夜变化已被证明会影响急性和慢性疾病的健康。关于昼夜节律和人类败血症之间的关系知之甚少。我们的目的是调查昼夜节律的变化，在人体内毒素血症模型的主机响应。一项交叉研究，12名健康年轻男性接受E。大肠杆菌内毒素（脂多糖，LPS）0.3 ng/kg，在中午12时，并在另一天，在午夜12时。分析血液样品的促炎细胞因子和抗炎细胞因子：肿瘤坏死因子（TNF）-α、可溶性TNF受体（sTNF-R）-1和-2、白细胞介素（IL）-1 β、IL-1受体拮抗剂（IL-1 Ra）、IL-6和IL-10以及YKL-40和氧化应激标志物丙二醛（MDA）、抗坏血酸（AA）和脱氢抗坏血酸（DHA），LPS给药后6和8小时。白天MDA和IL-10水平显着升高（P<0.05），而夜间TNF-α、sTNF-RI、sTNF-RII、IL-1 Ra、IL-6和YKL-40水平较高（P<0.01）所有比较）。AA和DHA的水平没有显着差异。健康志愿者对内毒素血症的急性期反应存在昼夜差异，夜间的炎症反应更明显。内毒素血症反应的昼夜节律差异可能在临床环境中发挥重要作用，应进一步研究。
Circadian variation in bodily functions has been shown to impact health in acute and chronic medical conditions. Little is known about the relationship between circadian rhythm and sepsis in humans. We aimed to investigate circadian variations in the host response in a human endotoxaemia model. A cross-over study, where 12 healthy young men received E. coli endotoxin (lipopolysaccharide, LPS) 0.3 ng/kg at 12 noon and, on another day, at 12 midnight. Blood samples were analysed for pro- and anti-inflammatory cytokines: tumour-necrosis factor (TNF)-alpha, soluble TNF receptors (sTNF-R)-1 and -2, interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1Ra), IL-6, and IL-10 as well as YKL-40 and the oxidative stress markers malondialdehyde (MDA), ascorbic acid (AA) and dehydroascorbic acid (DHA) before and at 2, 4, 6 and 8 hours after LPS administration. The levels of MDA and IL-10 where significantly higher during the day time (P<0.05) whereas levels of TNF-alpha, sTNF-RI, sTNF-RII, IL-1Ra, IL-6, and YKL-40 were higher (P<0.01 for all comparisons) during the night time. No significant differences were seen in the levels of AA and DHA. A day-night difference in the acute phase response to endotoxaemia exists in healthy volunteers with a more pronounced inflammatory response during the night time. This circadian difference in the response to endotoxaemia may play an important role in the clinical setting and should be investigated further.
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影响因子：
2.8
作者：
Cermakian N;Lange T;Golombek D;Sarkar D;Nakao A;Shibata S;Mazzoccoli G
通讯作者：
Mazzoccoli G
影响因子：
3.1
作者：
Hattori, Noriyuki;Oda, Shigeto;Hirasawa, Hiroyuki
通讯作者：
Hirasawa, Hiroyuki
影响因子：
4.6
作者：
Johansen, JS;Krabbe, KS;Pedersen, BK
通讯作者：
Pedersen, BK
影响因子：
4.6
作者：
Erikstrup, C;Ullum, H;Pedersen, BK
通讯作者：
Pedersen, BK
影响因子：
5.4
作者：
Hermann, C;von Aulock, S;Hartung, T
通讯作者：
Hartung, T