Autoinducer-2 is produced in saliva-fed flow conditions relevant to natural oral biofilms.
Autoinducer-2 is produced in saliva-fed flow conditions relevant to natural oral biofilms.
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DOI：
10.1111/j.1365-2672.2008.03910.x
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发表时间：
2008-12
影响因子：
4
通讯作者：
Kolenbrander PE
中科院分区：
文献类型：
--
作者：
Rickard AH;Campagna SR;Kolenbrander PE
我们评估了口腔细菌的双物种群落在唾液喂养的生物膜中产生通用信号分子自诱导物-2（AI-2）的能力。口腔链球菌34、S. oralis 34 luxS突变体和内氏放线菌T14 V作为单种和双种生物膜在用25%人唾液喂养的山梨醇中生长。通过哈氏弧菌BB 170生物发光测定法测定生物膜流出物中的AI-2浓度。在吸附山梨醇以释放生物膜细胞后，通过荧光抗体标记的细胞的荧光分析来确定细胞数量。48 h后，叉指状S. oralis 34和A. Naeslundii T14 V含有3.2 × 109个细胞，是单一物种生物膜的5倍。然而，这些48小时的双物种生物膜表现出最低的AI-2的浓度比细胞密度。口腔细菌在唾液喂养的生物膜中产生AI-2。在1 ~ 48 h内，S. oralis34-A. Naeslundii T14 V生物膜表明，AI-2的峰值产生发生较早，随后是非常低的稳态水平。高口腔细菌生物膜密度可以通过种间AI-2信号传导实现。我们认为，低浓度的AI-2有助于口腔黏膜生物膜群落的建立。
We evaluated the ability of a dual-species community of oral bacteria to produce the universal signalling molecule, autoinducer-2 (AI-2), in saliva-fed biofilms. Streptococcus oralis 34, S. oralis 34 luxS mutant and Actinomyces naeslundii T14V were grown as single- and dual-species biofilms within sorbarods fed with 25% human saliva. AI-2 concentration in biofilm effluents was determined by the Vibrio harveyi BB170 bioluminescence assay. After homogenizing the sorbarods to release biofilm cells, cell numbers were determined by fluorometric analysis of fluorescent antibody-labelled cells. After 48 h, dual-species biofilm communities of interdigitated S. oralis 34 and A. naeslundii T14V contained 3.2 × 109 cells: fivefold more than single-species biofilms. However, these 48-h dual-species biofilms exhibited the lowest concentration ratio of AI-2 to cell density. Oral bacteria produce AI-2 in saliva-fed biofilms. The decrease of more than 10-fold in concentration ratio seen between 1 and 48 h in S. oralis 34–A. naeslundii T14V biofilms suggests that peak production of AI-2 occurs early and is followed by a very low steady-state level. High oral bacterial biofilm densities may be achieved by inter-species AI-2 signalling. We propose that low concentrations of AI-2 contribute to the establishment of oral commensal biofilm communities.
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影响因子：
5.2
作者：
Semmelhack, MF;Campagna, SR;Bassler, BL
通讯作者：
Bassler, BL
影响因子：
3.1
作者：
CISAR, JO;KOLENBRANDER, PE;MCINTIRE, FC
通讯作者：
MCINTIRE, FC
DOI：
10.1111/j.1365-2672.1995.tb03128.x
发表时间：
1995-07-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
HODGSON, AE;NELSON, SM;GILBERT, P
通讯作者：
GILBERT, P
影响因子：
3.1
作者：
MCINTIRE, FC;VATTER, AE;ARNOLD, J
通讯作者：
ARNOLD, J
影响因子：
3.1
作者：
Shao, Hanjuan;Lamont, Richard J.;Demuth, Donald R.
通讯作者：
Demuth, Donald R.