The glutamatergic system in the preoptic area is involved in the retention of maternal behavior in maternally experienced female rats
The glutamatergic system in the preoptic area is involved in the retention of maternal behavior in maternally experienced female rats
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视前区的谷氨酸能系统参与母性经历过的雌性大鼠母性行为的保留
DOI：
10.1016/j.psyneuen.2020.104792
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发表时间：
2020
影响因子：
3.7
通讯作者：
Tsukahara Shinji
中科院分区：
文献类型：
--
作者：
Okino Eri;Morita Sayaka;Hoshikawa Yumi;Tsukahara Shinji
有母性经验的雌性大鼠在获得母性经验后，在很长一段时间内表现出较高的母性行为表现，尽管对母性行为保持的机制尚不清楚。内侧视前区(MPOA)在雌性大鼠母系行为的发生和维持中起重要作用。我们的目的是确定母性经历是否影响雌性大鼠母性行为保留的MPOA中的谷氨酸能系统。首先，为了确定产后经历对树突棘的影响，树突棘是兴奋性谷氨酸能神经传递的突触后成分，我们检测了经历过哺乳直到断奶的初产母亲(经验丰富的母亲)和在分娩当天与幼崽分离的初产母亲(经验不足的母亲)的树突棘数量。与经验不足的母亲相比，经验丰富的母亲的蘑菇刺数量明显更多，但其他类型的脊椎并不明显。接下来，为了确定产后母体经历对离子型谷氨酸受体表达的影响，我们检测了初产雌性大鼠的MPOA中AMPA受体亚单位(GluA1-A4)和NMDA受体亚单位(GluN1，GluN2a-2D)的mRNA水平，直到取脑。结果发现，初产雌性仔猪断奶当天GluA3和GluN2B基因的表达水平显著高于初产雌性仔猪。此外，我们还观察了AMPA受体拮抗剂CNQX和NMDA受体拮抗剂MK-801注入MPOA对初产雌性大鼠母体行为的影响。MPOA注射CNQX或MK-801后，母鼠的行为活动明显减少。这些发现表明，产后长期的母性经历通过增加蘑菇刺的数量和谷氨酸受体的表达而上调谷氨酸能神经传递，这可能与母性经历的雌性大鼠保持母性行为有关。
Maternally experienced female rats show high maternal behavior performance for a long time after acquisition of maternal experience, although the mechanisms responsible for the retention of maternal behavior are not well understood. The medial preoptic area (MPOA) plays an important role in the onset and maintenance of maternal behavior in female rats. We aimed to determine whether maternal experience affects the glutamatergic system in the MPOA for the retention of maternal behavior in female rats. First, to determine the effects of maternal experience in the postpartum period on dendritic spines, which are the postsynaptic component of excitatory glutamatergic neurotransmission, we examined the number of dendritic spines on MPOA neurons of primiparous mothers that had experienced mothering until weaning (sufficiently experienced mothers) and of primiparous mothers that were separated from their pups on the day of parturition (insufficiently experienced mothers). The number of mushroom spines, but not other types of spine, was significantly greater in the sufficiently experienced mothers compared with that in the insufficiently experienced mothers. Next, to determine the effects of maternal experience in the postpartum period on the expression of ionotropic glutamate receptors, we measured the mRNA levels of AMPA receptor subunits (GluA1–A4) and NMDA receptor subunits (GluN1, GluN2A–2D) in the MPOA of primiparous female rats that were kept with pups until brain sampling. As a result, we found that the mRNA levels of GluA3 and GluN2B were significantly higher in primiparous females on the day of weaning compared with those in primiparous females on the day of parturition. Additionally, we examined the effects of CNQX, an AMPA receptor antagonist, and MK-801, an NMDA receptor antagonist, injected into the MPOA on maternal behavior in maternally experienced primiparous female rats. Maternal behavioral activity was significantly reduced when CNQX or MK-801 was injected into the MPOA. These findings indicate that long-term maternal experience in the postpartum period up-regulates glutamatergic neurotransmission by increasing the number of mushroom spines and glutamate receptor expression, which may be involved in the retention of maternal behavior in maternally experienced female rats.
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影响因子：
1.9
作者：
Vigdorchik, Anna V.;Parrish, Bradley P.;Lagoda, Gwen A.;McHenry, Jenna A.;Hull, Elaine M.
通讯作者：
Hull, Elaine M.
影响因子：
3.7
作者：
J. Rosenblatt;A. D. Mayer;A. Giordano
通讯作者：
J. Rosenblatt;A. D. Mayer;A. Giordano
不同生殖状态下大鼠脑细胞内孕酮受体的表达及其对母性行为的影响
影响因子：
2.9
作者：
M. Numan;J. Roach;M. D. Cerro;A. Guillamón;S. Segovia;Teige P. Sheehan;M. J. Numan
通讯作者：
M. J. Numan
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影响因子：
3.4
作者：
M. R. Melis;S. Succu;M. Mascia;Luca Cortis;A. Argiolas
通讯作者：
A. Argiolas
影响因子：
4.8
作者：
BRIDGES, RS
通讯作者：
BRIDGES, RS