Cutaneous exposure to agglomerates of silica nanoparticles and allergen results in IgE-biased immune response and increased sensitivity to anaphylaxis in mice.
Cutaneous exposure to agglomerates of silica nanoparticles and allergen results in IgE-biased immune response and increased sensitivity to anaphylaxis in mice.
复制标题
皮肤暴露于二氧化硅纳米颗粒和过敏原的聚集体会导致IgE偏见的免疫反应，并增加对小鼠过敏反应的敏感性。
DOI：
10.1186/s12989-015-0095-3
复制
发表时间：
2015-06-26
影响因子：
10
通讯作者：
Tsutsumi Y
中科院分区：
文献类型：
--
作者：
Hirai T;Yoshioka Y;Takahashi H;Ichihashi K;Udaka A;Mori T;Nishijima N;Yoshida T;Nagano K;Kamada H;Tsunoda S;Takagi T;Ishii KJ;Nabeshi H;Yoshikawa T;Higashisaka K;Tsutsumi Y
皮肤是人类暴露于纳米材料的关键途径，这通常与暴露于其他化学和环境过敏原同时发生。然而，人们对通过皮肤接触纳米材料的危害知之甚少，特别是当同时接触其他物质时。用螨提取物和二氧化硅纳米颗粒的混合物重复局部处理NC/Nga小鼠的双耳和剃毛的上背部，NC/Nga小鼠是人类特应性皮炎（AD）的模型，诱导AD样皮肤病变。耳厚度测量和组织学分析显示，皮肤暴露于二氧化硅纳米粒子不会加重AD样皮肤病变。相反，同时皮肤暴露于螨过敏原和二氧化硅纳米颗粒导致低水平的过敏原特异性IgG的产生，包括Th 2相关的IgG 1和Th 1相关的IgG 2a亚型，过敏原特异性IgE浓度和Th 1和Th 2免疫应答的变化很少。此外，免疫反应的这些变化增加了对过敏反应的敏感性。当小鼠暴露于过敏原-二氧化硅纳米颗粒团聚体时，诱导低水平IgG产生，但当小鼠暴露于与过敏原分开施用的纳米颗粒或分散良好的纳米颗粒时，则不诱导低水平IgG产生。我们的数据表明，二氧化硅纳米粒子本身不直接影响过敏原特异性免疫反应后，同时局部应用纳米粒子和过敏原。然而，当存在于过敏原吸附的聚集体中时，二氧化硅纳米颗粒导致低IgG/IgE比率，这是人类特应性过敏的关键风险因素。我们认为，最大限度地减少纳米材料和过敏原之间的相互作用将增加纳米材料应用于皮肤的安全性。本文的在线版本（doi：10.1186/s12989-015-0095-3）包含补充材料，可供授权用户使用。
The skin is a key route of human exposure to nanomaterials, which typically occurs simultaneously with exposure to other chemical and environmental allergen. However, little is known about the hazards of nanomaterial exposure via the skin, particularly when accompanied by exposure to other substances. Repeated topical treatment of both ears and the shaved upper back of NC/Nga mice, which are models for human atopic dermatitis (AD), with a mixture of mite extract and silica nanoparticles induced AD-like skin lesions. Measurements of ear thickness and histologic analyses revealed that cutaneous exposure to silica nanoparticles did not aggravate AD-like skin lesions. Instead, concurrent cutaneous exposure to mite allergens and silica nanoparticles resulted in the low-level production of allergen-specific IgGs, including both the Th2-related IgG1 and Th1-related IgG2a subtypes, with few changes in allergen-specific IgE concentrations and in Th1 and Th2 immune responses. In addition, these changes in immune responses increased the sensitivity to anaphylaxis. Low-level IgG production was induced when the mice were exposed to allergen–silica nanoparticle agglomerates but not when the mice exposed to nanoparticles applied separately from the allergen or to well-dispersed nanoparticles. Our data suggest that silica nanoparticles themselves do not directly affect the allergen-specific immune response after concurrent topical application of nanoparticles and allergen. However, when present in allergen-adsorbed agglomerates, silica nanoparticles led to a low IgG/IgE ratio, a key risk factor of human atopic allergies. We suggest that minimizing interactions between nanomaterials and allergens will increase the safety of nanomaterials applied to skin. The online version of this article (doi:10.1186/s12989-015-0095-3) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
10
作者：
Ilves M;Palomäki J;Vippola M;Lehto M;Savolainen K;Savinko T;Alenius H
通讯作者：
Alenius H
影响因子：
14.2
作者：
Jutel, M;Jaeger, L;Cromwell, O
通讯作者：
Cromwell, O
DOI：
10.1164/rccm.200506-964oc
发表时间：
2006-07-01
影响因子：
24.7
作者：
Jeal, Hayley;Draper, Adrian;Jones, Meinir
通讯作者：
Jones, Meinir
影响因子：
7
作者：
Kawakami T;Ando T;Kimura M;Wilson BS;Kawakami Y
通讯作者：
Kawakami Y
影响因子：
78.8
作者：
Jain, Rakesh K.;Stylianopoulos, Triantafyllos
通讯作者：
Stylianopoulos, Triantafyllos