Assessing viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time.
Assessing viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time.
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DOI：
10.1038/srep11956
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发表时间：
2015-07-06
影响因子：
4.6
通讯作者：
Präbst K
中科院分区：
文献类型：
--
作者：
Taeger CD;Friedrich O;Dragu A;Weigand A;Hobe F;Drechsler C;Geppert CI;Arkudas A;Münch F;Buchholz R;Pollmann C;Schramm A;Birkholz T;Horch RE;Präbst K
当保存用于移植的组织时，预防缺血相关的细胞损伤是优先考虑的。灌注方案已被确立用于各种应用，并被证明上级临床常规中使用的程序。肌肉组织的体外灌注虽然繁琐，但由于其对缺血相关损伤高度敏感，因此是高度期望的。为了显示不同灌注协议的功效，可以使用外部场刺激来在活动和运行灌注协议期间立即可视化组织的改善或恶化。该方法已被用于显示使用肝素化盐水溶液灌注的猪腹直肌体外灌注的优越性。灌注的肌肉表现出统计学显着较高的能力，施加力相比，nonperfused的。这些发现可以使用膜联蛋白V作为细胞损伤的标志物来证实，与未灌注的组织相比，肌肉组织的灌注显著限制了损伤。体外灌注和外场刺激的结合可能会改善器官保护研究。
Preventing ischemia-related cell damage is a priority when preserving tissue for transplantation. Perfusion protocols have been established for a variety of applications and proven to be superior to procedures used in clinical routine. Extracorporeal perfusion of muscle tissue though cumbersome is highly desirable since it is highly susceptible to ischemia-related damage. To show the efficacy of different perfusion protocols external field stimulation can be used to immediately visualize improvement or deterioration of the tissue during active and running perfusion protocols. This method has been used to show the superiority of extracorporeal perfusion using porcine rectus abdominis muscles perfused with heparinized saline solution. Perfused muscles showed statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. The combination of extracorporeal perfusion and external field stimulation may improve organ conservation research.
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影响因子：
5.3
作者：
Horch RE;Boos AM;Quan Y;Bleiziffer O;Detsch R;Boccaccini AR;Alexiou C;Sun J;Beier JP;Arkudas A
通讯作者：
Arkudas A
影响因子：
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Arkudas A
影响因子：
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作者：
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影响因子：
82.9
作者：
Narula, J;Acio, ER;Strauss, HW
通讯作者：
Strauss, HW
影响因子：
2.3
作者：
Dragu, Adrian;Taeger, Christian D.;Praebst, Konstantin
通讯作者：
Praebst, Konstantin