Characterisation of the physical composition and microbial community structure of biofilms within a model full-scale drinking water distribution system.
Characterisation of the physical composition and microbial community structure of biofilms within a model full-scale drinking water distribution system.
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DOI：
10.1371/journal.pone.0115824
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Boxall JB
中科院分区：
文献类型：
--
作者：
Fish KE;Collins R;Green NH;Sharpe RL;Douterelo I;Osborn AM;Boxall JB
在饮用水配水系统(DWDS)中，微生物在管道内表面形成多种生物膜。细胞外聚合物(EPS)的基质是由附着的群落产生的，并为生物膜提供结构和稳定性。如果EPS的粘合强度下降或被外部剪切力克服，生物膜就会被动员到水中，可能会导致水质恶化。然而，由于获取生物膜样品的复杂性和分析EPS的困难，人们对DWDS生物膜内的EPS或群落组成或环境参数如何影响EPS知之甚少。此外，尽管生物膜可能包含各种微生物群，但研究通常只集中在细菌上。本研究应用基于荧光共聚焦激光扫描显微镜(CLSM)和数字图像分析(DIA)相结合的EPS分析方法，在具有典型水力条件的全尺寸DWDS实验管环装置中同时表征饮用水生物膜中的细胞和EPS(碳水化合物和蛋白质)。在DWDS实验设施中生长了28天后，从管道周围不同位置采集的生物膜应用了EPS分析方法，并对细菌、古菌和真菌群落进行了DNA指纹分析。EPS的体积是生物膜内细胞体积的4.9倍，其中碳水化合物是主要成分。此外，EPS的比例最大，位于细胞的上方。真菌和古菌被确定为生物膜群落的重要组成部分，尽管细菌更加多样化。此外，不同位置的生物膜在群落结构、细胞和EPS的数量、组成和三维分布方面都是相似的，表明管壁的活跃定植是DWDS内物质积累的重要驱动因素。
Within drinking water distribution systems (DWDS), microorganisms form multi-species biofilms on internal pipe surfaces. A matrix of extracellular polymeric substances (EPS) is produced by the attached community and provides structure and stability for the biofilm. If the EPS adhesive strength deteriorates or is overcome by external shear forces, biofilm is mobilised into the water potentially leading to degradation of water quality. However, little is known about the EPS within DWDS biofilms or how this is influenced by community composition or environmental parameters, because of the complications in obtaining biofilm samples and the difficulties in analysing EPS. Additionally, although biofilms may contain various microbial groups, research commonly focuses solely upon bacteria. This research applies an EPS analysis method based upon fluorescent confocal laser scanning microscopy (CLSM) in combination with digital image analysis (DIA), to concurrently characterize cells and EPS (carbohydrates and proteins) within drinking water biofilms from a full-scale DWDS experimental pipe loop facility with representative hydraulic conditions. Application of the EPS analysis method, alongside DNA fingerprinting of bacterial, archaeal and fungal communities, was demonstrated for biofilms sampled from different positions around the pipeline, after 28 days growth within the DWDS experimental facility. The volume of EPS was 4.9 times greater than that of the cells within biofilms, with carbohydrates present as the dominant component. Additionally, the greatest proportion of EPS was located above that of the cells. Fungi and archaea were established as important components of the biofilm community, although bacteria were more diverse. Moreover, biofilms from different positions were similar with respect to community structure and the quantity, composition and three-dimensional distribution of cells and EPS, indicating that active colonisation of the pipe wall is an important driver in material accumulation within the DWDS.
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影响因子：
2.7
作者：
Jahn, A;Nielsen, PH
通讯作者：
Nielsen, PH
影响因子：
12.8
作者：
Husband, P. S.;Boxall, J. B.;Saul, A. J.
通讯作者：
Saul, A. J.
影响因子：
4.3
作者：
Ivleva, Natalia P.;Wagner, Michael;Haisch, Christoph
通讯作者：
Haisch, Christoph
影响因子：
2.3
作者：
Buss, HL;Brantley, SL;Liermann, LJ
通讯作者：
Liermann, LJ
影响因子：
4.2
作者：
Gomez-Alvarez V;Revetta RP;Santo Domingo JW
通讯作者：
Santo Domingo JW