Fibronectin and vitronectin promote human fetal osteoblast cell attachment and proliferation on nanoporous titanium surfaces.
Fibronectin and vitronectin promote human fetal osteoblast cell attachment and proliferation on nanoporous titanium surfaces.
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DOI：
10.1166/jbn.2013.1601
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发表时间：
2013-06
影响因子：
2.9
通讯作者：
Ramirez-Vick JE
中科院分区：
文献类型：
--
作者：
Rivera-Chacon DM;Alvarado-Velez M;Acevedo-Morantes CY;Singh SP;Gultepe E;Nagesha D;Sridhar S;Ramirez-Vick JE
改善种植体生物材料的骨传导需要关注骨-种植体界面，这是一个复杂的多因素系统。种植体的表面形貌在该界面起着至关重要的作用。与骨植入材料的微结构化表面相比，纳米结构化表面已显示出促进血清蛋白吸附和成骨细胞粘附。我们研究了血清蛋白纤连蛋白和玻连蛋白对成骨细胞在纳米结构二氧化钛表面的附着和增殖的影响。人胎儿成骨细胞hFOB 1.19被用作模型成骨细胞，并在纳米多孔TiO 2模板上生长，使用Ti6 Al 4V和商业纯Ti基底作为对照。结果表明，在纳米多孔二氧化钛上的细胞增殖的显着增加在平坦的基板。初始细胞附着数据显示纤连蛋白或玻连蛋白对任何测试材料表面的细胞粘附具有显著影响。此外，细胞粘附的程度是显着不同的纳米多孔二氧化钛和两个Ti6 Al 4V和商业纯钛基板，与第一显示最高的表面覆盖。使用任何材料基底，纤维连接蛋白或玻连蛋白的存在对成骨细胞附着或增殖没有显著差异。两者合计，这些结果表明，在纳米多孔二氧化钛上显示的成骨细胞附着和增殖的增加是由于增加的纤维连接蛋白和玻连蛋白的吸附，因为更高的表面积和增强的蛋白质展开，这允许访问这些蛋白质内的成骨细胞结合基序。
Improvements in osteoconduction of implant biomaterials require focusing on the bone-implant interface, which is a complex multifactorial system. Surface topography of implants plays a crucial role at this interface. Nanostructured surfaces have been shown to promote serum protein adsorption and osteoblast adhesion when compared to microstructured surfaces for bone-implant materials. We studied the influence of the serum proteins fibronectin and vitronectin on the attachment and proliferation of osteoblasts onto nanostructured titania surfaces. Human fetal osteoblastic cells hFOB 1.19 were used as model osteoblasts and were grown on nanoporous TiO2 templates, using Ti6Al4V and commercially pure Ti substrates as controls. Results show a significant increase in cell proliferation on nanoporous TiO2 over flat substrates. Initial cell attachment data exhibited a significant effect by either fibronectin or vitronectin on cell adhesion at the surface of any of the tested materials. In addition, the extent of cell adhesion was significantly different between the nanoporous TiO2 and both Ti6Al4V and commercially pure Ti substrates, with the first showing the highest surface coverage. There was no significant difference on osteoblast attachment or proliferation between the presence of fibronectin or vitronectin using any of the material substrates. Taken together, these results suggest that the increase in osteoblast attachment and proliferation shown on the nanoporous TiO2 is due to an increase in the adsorption of fibronectin and vitronectin because of the higher surface area and to an enhanced protein unfolding, which allows access to osteoblast binding motifs within these proteins.
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影响因子：
14
作者：
García, AJ
通讯作者：
García, AJ
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