Validation of edible taste strips for identifying PROP taste recognition thresholds.
Validation of edible taste strips for identifying PROP taste recognition thresholds.
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DOI：
10.1002/lary.21757
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发表时间：
2011-06
期刊：
影响因子：
2.6
通讯作者：
Griffith, James W.
中科院分区：
文献类型：
--
作者：
Desai, Hetvi;Smutzer, Gregory;Coldwell, Susan E.;Griffith, James W.
目的/假设本研究的目的是验证使用可食用味觉条测量苦味化合物6-n-丙基硫氧嘧啶（PROP）的味觉识别阈值。研究设计通过两种不同的方法获得PROP的味觉识别阈值。在通过鼻子的气流被阻塞的受试者中也发现了阻塞。阈值，然后进行比较，基因型分析的TAS 2 R38味觉受体，这是主要的决定因素PROP.MethodsEdible味条检测PROP. Taste-recognition阈值的方法进行了测定的方法的上升限制和逆转。TAS 2 R38基因的单核苷酸多态性分析被用来确定PROP taster status.ResultsTaste识别阈值PROP形成两个分布。一组的平均浓度从4到219 nmol不等，代表PROP品尝者。第二组在≤800 nmol时无法检测到PROP的苦味，代表PROP非味觉者。极限上升法和反向法得出了类似的阈值结果。PAV等位基因的表达允许PROP的检测，但AVI纯合子不能检测到苦味PROP.ConclusionsEdible味道条成功地用于检测PROP阈值等于或低于那些在以前的研究中获得的PROP溶液或PROP浸渍滤纸。本研究为使用可食用味觉条识别人群中的PROP提供了有效性证据。
Objectives/HypothesisThe purpose of this study was to validate the use of edible taste strips for measuring taste recognition thresholds for the bitter‐tasting compound 6‐n‐propylthiouracil (PROP).Study DesignTaste recognition thresholds for PROP were obtained by two separate methods. Thresholds were also identified in subjects whose airflow through the nose was blocked. Threshold values were then compared to genotype analysis of the TAS2R38 taste receptor, which is the major determinant for the detection of PROP.MethodsEdible taste strips were used to examine taste recognition thresholds for PROP. Thresholds were determined by the method of ascending limits and by the method of reversals. Single nucleotide polymorphism analysis of theTAS2R38gene was used to identify PROP taster status.ResultsTaste recognition thresholds for PROP formed two distributions. Thresholds for one group varied from 4 to 219 nmol and represented PROP tasters. The second group could not detect the bitter taste of PROP at ≤800 nmol and represented PROP nontasters. The method of ascending limits and the method of reversals yielded similar threshold results. The expression of a PAV allele permitted detection of PROP, but AVI homozygotes could not detect the bitter taste of PROP.ConclusionsEdible taste strips were successfully used to detect PROP thresholds at values equal to or lower than those obtained in previous studies using PROP solutions or PROP‐impregnated filter papers. This study provides validity evidence for the use of edible taste strips for identifying PROP in the human population.
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