Optimization of Chondrocyte Viability in Partially Decellularized Tracheal Grafts.
Optimization of Chondrocyte Viability in Partially Decellularized Tracheal Grafts.
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DOI：
10.1002/ohn.404
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发表时间：
2023-11
影响因子：
3.4
通讯作者：
Chiang, Tendy Y.
中科院分区：
文献类型：
--
作者：
Bergman, Maxwell;Harwood, Jacqueline;Liu, Lumei;Dharmadikhari, Sayali;Shontz, Kimberly M. M.;Chiang, Tendy Y.
组织工程化气管置换（TETR）的进展显示，使用部分脱细胞气管移植物（PDTG）有望解决气道管理和重建的关键差距。在这项研究中，旨在利用软骨的免疫保护性质，以保持气管的生物力学，我们优化PDTG保留天然软骨细胞。小鼠体内比较研究。第三儿科医院附属研究所。使用十二烷基硫酸钠按照缩短的脱细胞方案制备PDTG，然后通过冷冻保存技术建立生物库。脱细胞效率通过DNA测定和组织学表征。用活/死和凋亡测定法评估植入前PDTG和生物库天然气管（对照）中软骨细胞的活力和凋亡。将PDTG（N = 5）和天然气管（N = 6）原位植入同基因受体1个月。在终点时，采用微型计算机断层扫描（micro-CT）来询问体内移植物通畅性和放射密度。血管化和上皮化在移植后使用组织学图像进行定性分析。与对照相比，PDTG表现出所有软骨外细胞的完全脱细胞化和降低的DNA含量。软骨细胞活力和非凋亡细胞群利用生物库和更短的脱细胞时间得到改善。所有移植物均保持通畅。1个月时移植物放射密度的评估显示，与宿主相比，PDTG和天然移植物的Hounsfield单位升高，PDTG显示出比天然移植物更高的放射密度。PDTG支持植入后1个月的完全上皮化和功能性再内皮化。优化PDTG软骨细胞的活力是成功的气管置换的关键组成部分。正在进行的研究旨在评估PDTG的急性和慢性免疫原性。
Advancements in tissue-engineered tracheal replacement (TETR) show promise for the use of partially decellularized tracheal grafts (PDTG) to address critical gaps in airway management and reconstruction. In this study, aiming to leverage the immunoprivileged nature of cartilage to preserve tracheal biomechanics, we optimize PDTG for retention of native chondrocytes. Comparison in vivo murine study. Research Institute affiliated with Tertiary Pediatric Hospital. PDTG were created per a shortened decellularization protocol using sodium dodecyl sulfate, then biobanked via cryopreservation technique. Decellularization efficiency was characterized by DNA assay and histology. Viability and apoptosis of chondrocytes in preimplanted PDTG and biobanked native trachea (control) was assessed with live/dead and apoptosis assays. PDTG (N = 5) and native trachea (N = 6) were orthotopically implanted in syngeneic recipients for 1-month. At the endpoint, microcomputed tomography (micro-CT) was employed to interrogate graft patency and radiodensity in vivo. Vascularization and epithelialization were qualitatively analyzed using histology images following explant. PDTG exhibited complete decellularization of all extra-cartilaginous cells and reduced DNA content compared to control. Chondrocyte viability and nonapoptotic cell populations were improved utilizing biobanking and shorter decellularization time. All grafts remained patent. Evaluation of graft radiodensity at 1 month revealed elevation of Hounsfield units in both PDTG and native compared to host, with PDTG showing higher radiodensity than native. PDTG supported complete epithelialization and functional reendothelialization 1-month postimplantation. Optimizing PDTG chondrocyte viability is a key component to successful tracheal replacement. Ongoing research seeks to evaluate the acute and chronic immunogenicity of PDTG.
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影响因子：
4.2
作者：
Liu, Y;Nakamura, T;Shimizu, Y
通讯作者：
Shimizu, Y
影响因子：
1.4
作者：
Dharmadhikari, Sayali;Best, Cameron A.;Chiang, Tendy
通讯作者：
Chiang, Tendy
DOI：
10.1007/978-1-61779-108-6_1
发表时间：
2011-01-01
期刊：
MAMMALIAN CELL VIABILITY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Stoddart, Martin J.
通讯作者：
Stoddart, Martin J.
DOI：
10.1016/s0022-5223(96)70366-5
发表时间：
1996-05-01
影响因子：
6
作者：
Yokomise, H;Inui, K;Hitomi, S
通讯作者：
Hitomi, S
影响因子：
3.4
作者：
Wilcox, Lyndy J.;Weber, Brittany C.;Baldassari, Cristina M.
通讯作者：
Baldassari, Cristina M.