Stem cell marker TRA-1-60 is expressed in foetal and adult kidney and upregulated in tubulo-interstitial disease.
Stem cell marker TRA-1-60 is expressed in foetal and adult kidney and upregulated in tubulo-interstitial disease.
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DOI：
10.1007/s00418-010-0741-7
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发表时间：
2010-10
影响因子：
2.3
通讯作者：
Collins, Jane
中科院分区：
文献类型：
--
作者：
Fesenko, Irina;Franklin, Danielle;Garnett, Paul;Bass, Paul;Campbell, Sara;Hardyman, Michelle;Wilson, David;Hanley, Neil;Collins, Jane
肾脏在受伤时具有自我修复的内在能力。远端小管上皮细胞可能参与再生。干细胞标志物TRA-1-60与人胚胎干细胞的多能性相关，并在分化时丢失。在人胎儿、成人和患病肾脏的连续切片中绘制并定量TRA-1-60表达。在8- 10周的人胎肾中，该表位在输尿管芽和由其衍生的结构（包括集合管上皮）上大量表达。在成人肾内髓质/乳头，与上皮膜抗原，水通道蛋白-2和Tamm-Horsfall蛋白的反应性的比较，证实了广泛的表达TRA-1-60的细胞内衬收集管和薄肢的亨利环，这可能是显着的，因为乳头被认为是港口慢循环细胞参与肾稳态和修复。在外髓质和皮质中，集合管和肾单位的管状细胞中存在罕见的零星表达，阳性细胞局限于细肢和粗升肢和远曲小管。值得注意的是，在显示肾小管间质损伤的皮质中，表达TRA-1-60的单个细胞和远端小管中的小细胞群的数量急剧增加。双重染色显示TRA-1-60阳性细胞共表达肾小管再生标志物Pax-2和Ki-67。考虑到胎儿肾脏中的定位和成人中的分布模式，推测TRA-1-60可能鉴定出有助于成人肾脏远端小管修复的细胞群是诱人的。
The kidney has an intrinsic ability to repair itself when injured. Epithelial cells of distal tubules may participate in regeneration. Stem cell marker, TRA-1-60 is linked to pluripotency in human embryonic stem cells and is lost upon differentiation. TRA-1-60 expression was mapped and quantified in serial sections of human foetal, adult and diseased kidneys. In 8- to 10-week human foetal kidney, the epitope was abundantly expressed on ureteric bud and structures derived therefrom including collecting duct epithelium. In adult kidney inner medulla/papilla, comparisons with reactivity to epithelial membrane antigen, aquaporin-2 and Tamm–Horsfall protein, confirmed extensive expression of TRA-1-60 in cells lining collecting ducts and thin limb of the loop of Henle, which may be significant since the papillae were proposed to harbour slow cycling cells involved in kidney homeostasis and repair. In the outer medulla and cortex there was rare, sporadic expression in tubular cells of the collecting ducts and nephron, with positive cells confined to the thin limb and thick ascending limb and distal convoluted tubules. Remarkably, in cortex displaying tubulo-interstitial injury, there was a dramatic increase in number of TRA-1-60 expressing individual cells and in small groups of cells in distal tubules. Dual staining showed that TRA-1-60 positive cells co-expressed Pax-2 and Ki-67, markers of tubular regeneration. Given the localization in foetal kidney and the distribution patterns in adults, it is tempting to speculate that TRA-1-60 may identify a population of cells contributing to repair of distal tubules in adult kidney.
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影响因子：
13.6
作者：
Cheng, HY;Lin, YY;Jou, TS
通讯作者：
Jou, TS
影响因子：
2.3
作者：
Gubhaju, Lina;Laslett, Andrew;Black, M. Jane
通讯作者：
Black, M. Jane
影响因子：
19.6
作者：
Knepper, MA;Wade, JB;Nielsen, S
通讯作者：
Nielsen, S
DOI：
10.1097/01.asn.0000067652.51441.21
发表时间：
2003-06-01
影响因子：
13.6
作者：
Bonventre, JV
通讯作者：
Bonventre, JV
影响因子：
3.8
作者：
Alvarez-Buylla, A;Seri, B;Doetsch, F
通讯作者：
Doetsch, F