Development and Application of Endothelial Cells Derived From Pluripotent Stem Cells in Microphysiological Systems Models.
Development and Application of Endothelial Cells Derived From Pluripotent Stem Cells in Microphysiological Systems Models.
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DOI：
10.3389/fcvm.2021.625016
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发表时间：
2021
影响因子：
3.6
通讯作者：
Truskey GA
中科院分区：
文献类型：
--
作者：
Kennedy CC;Brown EE;Abutaleb NO;Truskey GA
血管内皮存在于所有器官和血管中，促进身体不同器官系统中营养物质和废物的交换，并为健康的血管功能奠定基调。内皮具有机械敏感性，对血管内剪切应力的大小和时间波形作出反应。内皮功能障碍可导致动脉粥样硬化和其他疾病。在体外器官系统（如血脑屏障和组织工程血管）中模拟内皮功能和功能障碍，需要为这些生物医学工程应用提供内皮细胞（ECs）。很难找到具有上述功能或功能障碍的原发、易于再生的ECs。相比之下，人类多能干细胞（hPSCs）可以从感兴趣的捐赠者那里获得，并且几乎可以无限期地更新。在这篇综述中，我们重点介绍了如何利用体内血管内皮细胞发育的知识将诱导多能干细胞（iPSC）分化为内皮细胞。然后，我们描述了目前如何将ipsc衍生的内皮细胞用于器官功能和疾病的体外模型以及体内应用。
The vascular endothelium is present in all organs and blood vessels, facilitates the exchange of nutrients and waste throughout different organ systems in the body, and sets the tone for healthy vessel function. Mechanosensitive in nature, the endothelium responds to the magnitude and temporal waveform of shear stress in the vessels. Endothelial dysfunction can lead to atherosclerosis and other diseases. Modeling endothelial function and dysfunction in organ systems in vitro, such as the blood–brain barrier and tissue-engineered blood vessels, requires sourcing endothelial cells (ECs) for these biomedical engineering applications. It can be difficult to source primary, easily renewable ECs that possess the function or dysfunction in question. In contrast, human pluripotent stem cells (hPSCs) can be sourced from donors of interest and renewed almost indefinitely. In this review, we highlight how knowledge of vascular EC development in vivo is used to differentiate induced pluripotent stem cells (iPSC) into ECs. We then describe how iPSC-derived ECs are being used currently in in vitro models of organ function and disease and in vivo applications.
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影响因子：
10
作者：
Abaci, Hasan E.;Guo, Zongyou;Coffman, Abigail;Gillette, Brian;Lee, Wen-han;Sia, Samuel K.;Christiano, Angela M.
通讯作者：
Christiano, Angela M.
影响因子：
4.6
作者：
Bang S;Lee SR;Ko J;Son K;Tahk D;Ahn J;Im C;Jeon NL
通讯作者：
Jeon NL
影响因子：
11.8
作者：
Corada M;Nyqvist D;Orsenigo F;Caprini A;Giampietro C;Taketo MM;Iruela-Arispe ML;Adams RH;Dejana E
通讯作者：
Dejana E
DOI：
10.1161/atvbaha.114.303218
发表时间：
2014-11-01
影响因子：
8.7
作者：
Corada, Monica;Morini, Marco Francesco;Dejana, Elisabetta
通讯作者：
Dejana, Elisabetta
影响因子：
4.1
作者：
Crosby, Cody O.;Valliappan, Deepti;Zoldan, Janet
通讯作者：
Zoldan, Janet