Early ABA-stimulated maintenance of Cl− homeostasis by mepiquat chloride priming confers salt tolerance in cotton seeds
Early ABA-stimulated maintenance of Cl− homeostasis by mepiquat chloride priming confers salt tolerance in cotton seeds
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通过助壮素引发在 ABA 刺激下维持 Clâ 稳态，赋予棉籽耐盐性
DOI：
10.1016/j.cj.2020.08.004
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Yan GT
中科院分区：
文献类型：
--
作者：
Wang N;Wang XR;Zhang HH;Liu XH;Shi JB;Dong Q;Xu QH;Gui HP;Song MZ;Yan GT
氯化甲哌鎓（MC）引发缓解了盐胁迫对棉花（Gossypium hirsutum L.）种子萌发的影响，但其作用机制尚不清楚。我们发现，MC引发增加耐盐性，证明了种子活力和发芽率显着增加，并减轻盐毒性，通过减少发芽种子中的Cl−积累。同时，电生理实验表明，MC引发的种子表现出上级Cl−排斥能力在根尖。盐胁迫下脱落酸（阿坝）合成阻断剂氟啶酮可消除MC引发的这些有益作用。MC引发诱导的早期反应，驯化和胁迫，表明在初始暴露于盐胁迫期间迅速增加阿坝含量。转录组分析表明，MC引发诱导的差异表达的基因（DEG）在发芽种子阵列。萌发种子中最显著的变化是MC引发诱导的DEG编码阿坝生物合成，阿坝catalysts，和阿坝信号通路的表达增加盐胁迫下。MC引发还增加了萌发种子中编码Cl−离子转运蛋白的一些DEG（例如CCC、SLAC 1/SLAH 1/SLAH 3、CLC和ALMT 9）的表达。这些结果表明，MC引发诱导的阿坝有助于组织中的Cl−稳态，并通过调节Cl−转运蛋白（特别是CC和SLAC 1/SLAH 1/SLAH 3）作为耐盐性的正调节剂。综上所述，这些发现揭示了MC介导的种子萌发过程中耐盐胁迫的分子机制。
Mepiquat chloride (MC) priming alleviates the effects of salt stress during seed germination in cotton (Gossypium hirsutumL.), but the mechanisms underlying its effects are unknown. We found that MC priming increases salt tolerance, as evidenced by marked increases in seed vigor and germination rates, and alleviated salt toxicity by reducing Cl−accumulation in germinating seeds. Consistently, electrophysiological experiments revealed that the seeds with MC priming displayed superior Cl−exclusion ability in the root apex. These beneficial effects of MC priming were abolished by the abscisic acid (ABA)-synthesis blocker fluridone under salt stress. MC priming induced an early response to acclimatization and stress, as indicated by rapidly increasing ABA content during initial exposure to salt stress. Transcriptome analyses revealed that MC priming induced an array of differentially expressed genes (DEGs) in germinating seeds. The most noticeable changes in germinating seeds were MC priming-induced increases in the expression of DEGs encoding components of ABA biosynthesis, ABA catabolism, and ABA signaling pathways under salt stress. MC priming also increased the expression of some DEGs encoding Cl−ion transporters (e.g.CCC,SLAC1/SLAH1/SLAH3,CLC, andALMT9) in germinating seeds. These results indicate that MC priming-induced ABA contributes to Cl−homeostasis in tissues and acts as a positive regulator of salt tolerance via regulation of Cl−transporters (particularlyCCCandSLAC1/SLAH1/SLAH3). Taken together, these findings shed light on the molecular mechanism underlying MC-mediated tolerance to salt stress during seed germination.
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DOI：
10.1016/j.plaphy.2020.05.021
发表时间：
2020-05
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
Lin Wang;Jia-yang Xu;W. Jia;Zheng Chen;Zicheng Xu
通讯作者：
Lin Wang;Jia-yang Xu;W. Jia;Zheng Chen;Zicheng Xu
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
褪黑激素通过 NADPH 氧化酶依赖性控制质膜 K 转运蛋白和 K 稳态来提高水稻盐胁迫耐受性
影响因子：
7.3
作者：
Liu, Juan;Shabala, Sergey;Zhao, Quanzhi
通讯作者：
Zhao, Quanzhi
DOI：
--
发表时间：
2015
期刊：
--
影响因子：
--
作者：
H. Nabizadeh;M. Valizadeh;M. Norouzi;M. Toorchi;Mohammad Behrouzi Vajovi
通讯作者：
H. Nabizadeh;M. Valizadeh;M. Norouzi;M. Toorchi;Mohammad Behrouzi Vajovi
影响因子：
5.6
作者：
Wei P;Wang L;Liu A;Yu B;Lam HM
通讯作者：
Lam HM